Mouse TNF-α DTSet enzyme-linked immunoassay kit
概述
产品名称
Mouse TNF-α DTSet enzyme-linked immunoassay kit
产品类型
DTSet Elisa Kit
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0209 | 0.0235 | 0.0222 | |
| 31.25 | 0.1188 | 0.1202 | 0.1195 | 0.0973 |
| 62.50 | 0.2134 | 0.2100 | 0.2117 | 0.1895 |
| 125.00 | 0.3996 | 0.4101 | 0.4049 | 0.3827 |
| 250.00 | 0.7716 | 0.7497 | 0.7607 | 0.7385 |
| 500.00 | 1.3720 | 1.3740 | 1.3730 | 1.3508 |
| 1000.00 | 2.1950 | 2.2010 | 2.1980 | 2.1758 |
| 2000.00 | 3.2350 | 3.1840 | 3.2095 | 3.1873 |
实验类型
夹心酶联免疫吸附测定(Sandwich Elisa)
种属反应性
Mouse
检测方法
比色法
实验检测时长
120min
标准曲线范围
31.25 pg/ml-2000 pg/ml
标准曲线梯度
7点/3倍
孵育次数
2
可检测样本
可溶性物质的液相样品。例如:血清,血浆,细胞培养上清,组织研磨液等。
加样体积
10 μl
产品特性
- 优化的捕获和检测抗体配对与推荐浓度可节省较长的开发时间
- 提供开发方案以指导进一步的分析优化
- 检测可根据您的特定需求进行定制
- 完整试剂盒的经济替代品
试剂盒组分
- 捕获抗体
- 检测抗体
- 重组标准品
- 与辣根过氧化物酶偶联的链霉亲和素 (Streptavidin-HRP)
所需的其他材料
DTSet辅助试剂盒(5板):包含96孔微孔板、封板膜、显色液、终止溶液、包板缓冲液(PBS)、洗涤缓冲液和检测缓冲液。
上面列出的部件可以单独购买:
- 96孔微孔板板(EP): YOUKE Life, Catalog # DSEP01
- 封板木膜(PS): YOUKE Life, Catalog # DSSF01。
- 包板缓冲液(CB): 137mM NaCl,2.7mM KCl,8.1mM Na2HPO4,1.5mM KH2PO4,pH 7.2-7.4,0.2m过滤。YOUKE Life, Catalog # DSCB01。
- 检测缓冲液 10X(AB): 0.5%BSA,0.05%Tween20,PBS溶液。YOUKE Life, Catalog # DSAB01。
- 封闭液(BB): YOUKE Life, Catalog # DSBB01。
- Washing Solution: 0.05%Tween20,PBS溶液,pH 7.2-7.4。YOUKE Life, Catalog # DSWB01。
- 底物显色液(TMB): TMB。YOUKE Life, Catalog # DSTS01。
- 终止溶液(SS): 0.5mol/ml H2SO4。YOUKE Life, Catalog # DSSS01。
靶点
UNIPROT
功能
肿瘤坏死因子α(TNF-α),又称恶病质和TNFSF1A,是TNF超家族的原型配体。它是一种多效性分子,在炎症、免疫系统发育、细胞凋亡和脂质代谢中起核心作用。TNF-α还参与许多病理状况,包括哮喘、克罗恩病、类风湿性关节炎、神经性疼痛、肥胖、2型糖尿病、感染性休克、自身免疫和癌症。
小鼠 TNF-α 合成为 26 kDa II 型跨膜蛋白,由 35 个氨基酸 (aa) 细胞质结构域、21个氨基酸跨膜段和 179个氨基酸细胞外结构域 (ECD) 组成。在ECD中,小鼠TNF-α与大鼠共享95%的氨基酸序列同一性,与犬、马、猫、人类、兔和猪TNF-α共享80%的氨基酸序列同一性。它由多种免疫细胞、上皮细胞、内皮细胞和肿瘤细胞产生。TNF-α在细胞内组装以形成在细胞表面表达的非共价连接的同源三聚体。细胞表面TNF-α既能诱导肿瘤细胞和病毒感染细胞的裂解,又能在可溶性TNF RI连接后产生自身的下游细胞信号传导。TACE/ADAM17脱落膜结合的TNF-α释放生物活性细胞因子,一种含有TNF-α细胞外结构域的55 kDa可溶性三聚体。
TNF-α结合无处不在的55-60 kDa TNF RI和造血细胞限制的78-80 kDa TNF RII,两者都也表达为同源三聚体。I型和II型受体都以相当的亲和力结合TNF-α,并且可以促进NFkB活化。然而,只有TNF RI含有触发细胞凋亡激活的细胞质死亡结构域。两种受体的可溶性形式都释放到人血清和尿液中,可以中和TNF的生物活性。
小鼠 TNF-α 合成为 26 kDa II 型跨膜蛋白,由 35 个氨基酸 (aa) 细胞质结构域、21个氨基酸跨膜段和 179个氨基酸细胞外结构域 (ECD) 组成。在ECD中,小鼠TNF-α与大鼠共享95%的氨基酸序列同一性,与犬、马、猫、人类、兔和猪TNF-α共享80%的氨基酸序列同一性。它由多种免疫细胞、上皮细胞、内皮细胞和肿瘤细胞产生。TNF-α在细胞内组装以形成在细胞表面表达的非共价连接的同源三聚体。细胞表面TNF-α既能诱导肿瘤细胞和病毒感染细胞的裂解,又能在可溶性TNF RI连接后产生自身的下游细胞信号传导。TACE/ADAM17脱落膜结合的TNF-α释放生物活性细胞因子,一种含有TNF-α细胞外结构域的55 kDa可溶性三聚体。
TNF-α结合无处不在的55-60 kDa TNF RI和造血细胞限制的78-80 kDa TNF RII,两者都也表达为同源三聚体。I型和II型受体都以相当的亲和力结合TNF-α,并且可以促进NFkB活化。然而,只有TNF RI含有触发细胞凋亡激活的细胞质死亡结构域。两种受体的可溶性形式都释放到人血清和尿液中,可以中和TNF的生物活性。
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