Mouse CD68/SR-D1 DTSet enzyme-linked immunoassay kit
Catalog# DSEM004205；DSEM004215
Mouse CD68/SR-D1 DTSet enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Mouse
概述
产品名称
Mouse CD68/SR-D1 DTSet enzyme-linked immunoassay kit
产品类型
DTSet Elisa Kit
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0520 | 0.0491 | 0.0506 | |
| 78.13 | 0.3821 | 0.3865 | 0.3843 | 0.3338 |
| 156.25 | 0.6670 | 0.6833 | 0.6752 | 0.6246 |
| 312.50 | 1.1680 | 1.1940 | 1.1810 | 1.1305 |
| 625.00 | 2.0140 | 2.0270 | 2.0205 | 1.9700 |
| 1250.00 | 3.0770 | 2.9900 | 3.0335 | 2.9830 |
| 2500.00 | 3.9851 | 4.0014 | 3.9933 | 3.9427 |
| 5000.00 | 4.6085 | 4.7459 | 4.6772 | 4.6267 |
实验类型
夹心酶联免疫吸附测定（Sandwich Elisa）
种属反应性
Mouse
检测方法
比色法
实验检测时长
120min
标准曲线范围
78.13pg/ml-5000pg/ml
标准曲线梯度
7点/3倍
孵育次数
2
可检测样本
可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。
加样体积
10 μl
产品特性
- 优化的捕获和检测抗体配对与推荐浓度可节省较长的开发时间
- 提供开发方案以指导进一步的分析优化
- 检测可根据您的特定需求进行定制
- 完整试剂盒的经济替代品
试剂盒组分
- 捕获抗体
- 检测抗体
- 重组标准品
- 与辣根过氧化物酶偶联的链霉亲和素 （Streptavidin-HRP）
所需的其他材料
DTSet辅助试剂盒（5板）：包含96孔微孔板、封板膜、显色液、终止溶液、包板缓冲液（PBS）、洗涤缓冲液和检测缓冲液。
上面列出的部件可以单独购买：
- 96孔微孔板板(EP): YOUKE Life, Catalog # DSEP01
- 封板木膜(PS): YOUKE Life, Catalog # DSSF01。
- 包板缓冲液(CB): 137mM NaCl，2.7mM KCl，8.1mM Na2HPO4，1.5mM KH2PO4，pH 7.2-7.4，0.2m过滤。YOUKE Life, Catalog # DSCB01。
- 检测缓冲液 10X(AB): 0.5%BSA，0.05%Tween20，PBS溶液。YOUKE Life, Catalog # DSAB01。
- 封闭液(BB): YOUKE Life, Catalog # DSBB01。
- Washing Solution: 0.05%Tween20，PBS溶液，pH 7.2-7.4。YOUKE Life, Catalog # DSWB01。
- 底物显色液(TMB): TMB。YOUKE Life, Catalog # DSTS01。
- 终止溶液(SS): 0.5mol/ml H2SO4。YOUKE Life, Catalog # DSSS01。
靶点
UNIPROT
功能
CD68，也称为清道夫受体 D1 （SR-D1），是一种 I 型跨膜糖蛋白，属于 LAMP 分子家族。CD68 在单核细胞和巨噬细胞上表达，可作为氧化 LDL 的清除剂受体。
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