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ICAM1 Recombinant Rabbit Monoclonal Antibody [ST0487]

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Catalog# ET1609-46

ICAM1 Recombinant Rabbit Monoclonal Antibody [ST0487]

Application

  • WB

  • IF-Tissue

  • IHC-P

Reactivity

  • Human

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

ICAM1 Recombinant Rabbit Monoclonal Antibody [ST0487]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within human ICAM1 aa 30-70.

种属反应性

Human

验证应用

WB, IF-Tissue, IHC-P

分子量

Predicted band size: 58 kDa

阳性对照

HepG2 cell lysate, Ramos cell lysate, Raji cell lysate, human liver tissue lysate, human colon cancer tissue, human lung cancer tissue, human kidney tissue, human lymph node tissue.

偶联

unconjugated

克隆号

ST0487

RRID

AB_3069856

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000-1:5,000

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:200

靶点

功能

Cell adhesion molecules (CAMs) are a family of closely related cell surface glycoproteins involved in cell-cell interactions during growth and are thought to play important, yet separate, roles in embryogenesis and development. The intracellular adhesion molecule-1 (ICAM-1), also referred to as CD54, is an integral membrane protein of the immunoglobulin superfamily and recognizes the beta2alpha1 and beta2alphaM Integrins. ICAM-2 functions as a ligand for lymphocyte function-associated antigen-1 (LFA-1) and is involved in leukocyte adhesion. ICAM-3 is highly expressed on the surface of human eosinophils and, when bound to ligand, may inhibit eosinophil inflammatory responses and survival. ICAM-4, also known as LW glycoprotein, interacts with Integrins alphaLbeta2, alphaMbeta2, alpha4beta1, the alphaV family and alphaIIbbeta3, and selective binding to different integrins may be relevant to the pathology in a number of red blood cell associated diseases. Lastly, ICAM-5, expressed on telencephalic neurons, binds CD11a/CD18 and thus may act as an adhesion molecule for leukocyte binding in the central nervous system.

背景文献

1. Grover JR et al. Basic motifs target PSGL-1, CD43, and CD44 to plasma membrane sites where HIV-1 assembles. J Virol 89:454-67 (2015).

2. Rouault C et al. Roles of chemokine ligand-2 (CXCL2) and neutrophils in influencing endothelial cell function and inflammation of human adipose tissue. Endocrinology 154:1069-79 (2013).

序列相似性

Belongs to the immunoglobulin superfamily. ICAM family.

翻译后修饰

Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.

亚细胞定位

Membrane.

UNIPROT

SwissProt: P05362 Human

别名

Antigen identified by monoclonal antibody

BB2 antibody

BB 2 antibody

BB2 antibody

CD 54 antibody

CD_antigen=CD54 antibody

CD54 antibody

Cell surface glycoprotein P3.58 antibody

Human rhinovirus receptor antibody

ICAM 1 antibody

展开

图片

  • Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (<a href="/products/ET1609-46" style="font-weight: bold;text-decoration: underline;">ET1609-46</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (20 µg/Lane)<br />Lane 2: Ramos cell lysate (20 µg/Lane)<br />Lane 3: Raji cell lysate (20 µg/Lane)<br />Lane 4: human liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 58 kDa<br />Observed band size: 110 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1609-46" style="font-weight: bold;text-decoration: underline;">ET1609-46</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (ET1609-46) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate (20 µg/Lane)
    Lane 2: Ramos cell lysate (20 µg/Lane)
    Lane 3: Raji cell lysate (20 µg/Lane)
    Lane 4: human liver tissue lysate (40 µg/Lane)

    Predicted band size: 58 kDa
    Observed band size: 110 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-46) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (<a href="/products/ET1609-46" style="font-weight: bold;text-decoration: underline;">ET1609-46</a>) at 1/2,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: K-562 cell lysate (low expression)<br />Lane 3: Raji cell lysate<br />Lane 4: Ramos cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 58 kDa<br />Observed band size: 110 kDa<br /><br />Exposure time: 42 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1609-46" style="font-weight: bold;text-decoration: underline;">ET1609-46</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (ET1609-46) at 1/2,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: K-562 cell lysate (low expression)
    Lane 3: Raji cell lysate
    Lane 4: Ramos cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 58 kDa
    Observed band size: 110 kDa

    Exposure time: 42 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-46) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-ICAM1 antibody (<a href="/products/ET1609-46" style="font-weight: bold;text-decoration: underline;">ET1609-46</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-46" style="font-weight: bold;text-decoration: underline;">ET1609-46</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-ICAM1 antibody (ET1609-46) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-46) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-ICAM1 antibody (<a href="/products/ET1609-46" style="font-weight: bold;text-decoration: underline;">ET1609-46</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-46" style="font-weight: bold;text-decoration: underline;">ET1609-46</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-ICAM1 antibody (ET1609-46) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-46) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ICAM1 antibody (<a href="/products/ET1609-46" style="font-weight: bold;text-decoration: underline;">ET1609-46</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-46" style="font-weight: bold;text-decoration: underline;">ET1609-46</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ICAM1 antibody (ET1609-46) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-46) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-ICAM1 antibody (<a href="/products/ET1609-46" style="font-weight: bold;text-decoration: underline;">ET1609-46</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-46" style="font-weight: bold;text-decoration: underline;">ET1609-46</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-ICAM1 antibody (ET1609-46) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-46) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
  2. IHC-P
  3. IF

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