ICAM1 Recombinant Rabbit Monoclonal Antibody [PSH09-31]
Catalog# HA723089
ICAM1 Recombinant Rabbit Monoclonal Antibody [PSH09-31]
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WB
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IHC-P
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IF-Tissue
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Human
概述
产品名称
ICAM1 Recombinant Rabbit Monoclonal Antibody [PSH09-31]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human ICAM1 aa 1-480.
种属反应性
Human
验证应用
WB, IHC-P, IF-Tissue
分子量
Predicted band size: 58 kDa
阳性对照
HepG2 cell lysate, K-562 cell lysate, Raji cell lysate, Ramos cell lysate, human colon cancer tissue, human kidney tissue, human lymph node tissue.
偶联
unconjugated
克隆号
PSH09-31
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:5,000
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IHC-P
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1:8,000
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IF-Tissue
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1:1,000
靶点
功能
ICAM-1 (Intercellular Adhesion Molecule 1) also known as CD54 (Cluster of Differentiation 54) is a protein that in humans is encoded by the ICAM1 gene. This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by rhinovirus as a receptor for entry into respiratory epithelium. The protein encoded by this gene is a type of intercellular adhesion molecule continuously present in low concentrations in the membranes of leukocytes and endothelial cells. Upon cytokine stimulation, the concentrations greatly increase. ICAM-1 can be induced by interleukin-1 (IL-1) and tumor necrosis factor (TNF) and is expressed by the vascular endothelium, macrophages, and lymphocytes. ICAM-1 is a ligand for LFA-1 (integrin), a receptor found on leukocytes. When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 and then transmigrate into tissues. LFA-1 has also been found in a soluble form, which seems to bind and block ICAM-1.
背景文献
1. Bai X et al. CDK4/6 inhibition triggers ICAM1-driven immune response and sensitizes LKB1 mutant lung cancer to immunotherapy. Nat Commun. 2023 Mar
2. Zhou Q et al. Role of ICAM1 in tumor immunity and prognosis of triple-negative breast cancer. Front Immunol. 2023 Aug
亚细胞定位
Membrane.
UNIPROT
别名
Antigen identified by monoclonal antibody BB2 antibody
BB 2 antibody
BB2 antibody
CD 54 antibody
CD_antigen=CD54 antibody
CD54 antibody
Cell surface glycoprotein P3.58 antibody
Human rhinovirus receptor antibody
ICAM 1 antibody
ICAM-1 antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (HA723089) at 1/5,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: K-562 cell lysate (low expression)
Lane 3: Raji cell lysate
Lane 4: Ramos cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 110 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723089) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (HA723089) at 1/10,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-ICAM1 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 100 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723089) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-ICAM1 antibody (HA723089) at 1/8,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723089) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ICAM1 antibody (HA723089) at 1/8,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723089) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-ICAM1 antibody (HA723089) at 1/8,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723089) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-Tissue
Species: Human
Site: colon cancer
Sample: Paraffin-embedded section
Antibody concentration: 1/1,000
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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