Exosomal Marker Antibody Kit
概述
试剂盒组分
产品包括 | 规格 | 应用 | 反应性 | MW(kDa) |
---|---|---|---|---|
ALIX[ET1705-74] | 20µl | WB,IHC-P,FC | Human,Rat,Mouse | Predicted band size: 96 kDa |
Annexin V[ET1702-62] | 20µl | WB,FC | Human,Mouse,Rat | Predicted band size: 36 kDa |
ICAM1[ET1609-46] | 20µl | WB,IF-Cell,IF-Tissue,IHC-P | Human | Predicted band size: 58 kDa |
CD9[ET1601-9] | 20µl | WB,IF-Cell,IF-Tissue,IHC-P,IP | Human,Mouse,Rat | Predicted band size: 25 kDa |
GM130 (cis-Golgi Marker)[R1608-7] | 20µl | WB,IHC-P,FC | Human,Mouse,Rat | Predicted band size: 113 kDa |
EpCAM[ET1608-26] | 20µl | WB,IHC-P | Human,Mouse,Rat | Predicted band size: 35 kDa |
Hsp70[ET1601-11] | 20µl | WB,IF-Cell,IF-Tissue,IHC-P,FC | Human,Mouse,Rat | Predicted band size: 70 kDa |
Flotillin 1[ET7107-82] | 20µl | WB,IHC-P,FC | Human,Rat,Mouse | Predicted band size: 47 kDa |
HRP Conjugated Alpaca anti-Rabbit IgG FC, Recombinant VHH[HA1031] | 100µl | IP,ELISA,IHC-P,WB | Rabbit |
产品描述
The Exosomal Marker Antibody Sampler Kit provides an economical method to evaluate the presence of exosome markers. The kit contains enough primary antibodies to perform two Western blotting experiments on each target.And also includes secondary reagent for detection of these antibodies.
产品特性
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
背景
Exosomes are small membrane-bound vesicles that in recent years have emerged as important molecules for inter-cellular communication.The exosomal markers contained in this kit are Alix, Annexin V, ICAM-1, CD9, GM130, EpCAM, flotillin, and HSP70.</br> Alix, a cytosolic scaffold protein, regulates many cellular processes including endocytic membrane trafficking, cell adhesion . Annexin V is a ~30 kDa protein that binds to phospho-lipids in a calcium-dependent manner. Intracellular cell adhesion molecule-1 (CD54 or ICAM-1) is a cell surface glycoprotein that belongs to the immunoglobulin superfamily (IgSF) of adhesion molecules.The CD9 antigen belongs to the tetraspanin family of cell surface glycoproteins. GM130 is required for membrane fusion events that mediate ribbon formation during Golgi assembly. Epithelial cell adhesion and activation molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates calcium-independent, hemophilic adhesions on the basolateral surface of most epithelial cells. Flotillins belong to a famiy of lipid raft-associated integral membrane proteins that are ubiquitously expressed and located to lipid rafts on the cell plasma membrane where they support signal transduction and regulate lipid raft motility and localization. HSP70 is a molecular chaperone expressed constituitively under normal conditions to maintain protein homeostatis and is induced upon environmental stress .
数据链接
SwissProt: Q9WU78 Mouse
SwissProt: Q9QZA2 Rat
SwissProt: P08758 Human
SwissProt: P48036 Mouse
SwissProt: P14668 Rat
SwissProt: P05362 Human
SwissProt: P21926 Human
SwissProt: P40240 Mouse
SwissProt: P40241 Rat
SwissProt: Q08379 Human
SwissProt: Q921M4 Mouse
SwissProt: Q62839 Rat
SwissProt: P16422 Human
SwissProt: Q99JW5 Mouse
SwissProt: O55159 Rat
SwissProt: P0DMV9 Human
SwissProt: P0DMV8 Human
SwissProt: P17879 Mouse
SwissProt: Q61696 Mouse
SwissProt: Q07439 Rat
SwissProt: O75955 Human
SwissProt: O08917 Mouse
SwissProt: Q9Z1E1 Rat
背景文献
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8. Théry, C. et al. (1999) J Cell Biol 147, 599-610.
9. Puthenveedu, M.A. et al. (2006) Nat Cell Biol 8, 238-48.
10. Barr, F.A. and Short, B. (2003) Curr Opin Cell Biol 15, 405-13.
11. Went, P.T. et al. (2004) Hum Pathol 35, 122-8.
12. Baeuerle, P.A. and Gires, O. (2007) Br J Cancer 96, 417-23.
13. Armstrong, A. and Eck, S.L. Cancer Biol Ther 2, 320-6.
14. Langhorst, M.F. et al. (2005) Cell Mol Life Sci 62, 2228-40.
15. Stuermer, C.A. and Plattner, H. (2005) Biochem Soc Symp , 109-18.
16. Fernow, I. et al. (2007) Eur J Cell Biol 86, 345-52.
17. Neumann-Giesen, C. et al. (2007) J Cell Sci 120, 395-406.
18. Liu, J. et al. (2005) J Biol Chem 280, 16125-34.
19. Santamaría, A. et al. (2005) Mol Cell Biol 25, 1900-11.
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21. Young, J.C. et al. (2003) Trends Biochem Sci 28, 541-7.
图片
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Western blot analysis of ALIX on different lysates with Rabbit anti-ALIX antibody (ET1705-74) at 1/2,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: K-562 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: PC-3M cell lysate
Lane 5: MCF7 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 96 kDa
Observed band size: 100 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-74) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of CD9 on different lysates with Rabbit anti-CD9 antibody (ET1601-9) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: MCF7 cell lysate (20 µg/Lane)
Lane 4: HCT 116 cell lysate (12 µg/Lane)
Lane 5: HepG2 cell lysate (20 µg/Lane)
Lane 6: Jurkat cell lysate (20 µg/Lane)
Lane 7: Raji cell lysate (20 µg/Lane)
Lane 8: Rat lung tissue lysate (20 µg/Lane)
Predicted band size: 25 kDa
Observed band size: 23 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-9) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Hsp70 on different lysates with Rabbit anti-Hsp70 antibody (ET1601-11) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: HCT 116 cell lysate
Lane 5: Mouse brain tissue lysate
Lane 6: Mouse testis tissue lysate
Lane 7: Rat testis tissue lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: Lane 1-4: 43 seconds; Lane 5-7: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-11) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.