Flotillin 1 Recombinant Rabbit Monoclonal Antibody [JB19-45]
Catalog# ET7107-82
Flotillin 1 Recombinant Rabbit Monoclonal Antibody [JB19-45]
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WB
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IHC-P
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FC
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Human
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Rat
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Mouse
概述
产品名称
Flotillin 1 Recombinant Rabbit Monoclonal Antibody [JB19-45]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Flotillin 1 aa 378-427 / 427.
种属反应性
Human, Rat, Mouse
验证应用
WB, IHC-P, FC
分子量
Predicted band size: 47 kDa
阳性对照
PC-3M, rat brain tissue, human lung cancer tissue, human liver tissue, human spleen tissue, HUVEC.
偶联
unconjugated
克隆号
JB19-45
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:1,000
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IHC-P
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1:50-1:100
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FC
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1:50-1:100
靶点
功能
Lipid rafts are sphingolipid- and cholesterol-rich membrane microdomains that are insoluble in nonionic detergents. Lipid rafts are important for numerous cellular processes, including signal transduction, membrane trafficking and molecular sorting. Flotillins are lipid raft components in neurons and caveloae-associated proteins in A498 kidney cells. Flotillin-1 belongs to the band 7.2/stomatin protein family, whose members are characterized by the presence of a hydrophobic N-terminal region that is predicted to form a single, outside to inside, transmembrane domain. Flotillin-1 and -2 have complementary tissue distributions and their expression levels are independently regulated. At the cellular level, Flotillin-2 is ubiquitously expressed, whereas Flotillin-1 is expressed in A498 kidney cells, muscle cell lines and fibroblasts. Flotillins form a ternary complex with CAP and Cbl, directing the localization of the CAP-Cbl complex to a lipid raft subdomain of the plasma membrane. Association of ER-X with Flotillin localizes ER-X within plasma membrane caveloae and mediates rapid oestrogen activation of the MAP kinase cascade. The expression of the flotillins is also correlated to the progression of Alzhemier pathology.
背景文献
1. Gorbea C et al. A protein interaction network for Ecm29 links the 26 S proteasome to molecular motors and endosomal components. J Biol Chem 285:31616-31633 (2010).
2. Chi A et al. Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes. J Proteome Res 5:3135-3144 (2006).
序列相似性
Belongs to the band 7/mec-2 family. Flotillin subfamily.
亚细胞定位
Cell membrane. Endosome.
UNIPROT #
别名
FLOT 1 antibody
FLOT1 antibody
FLOT1_HUMAN antibody
Flotillin-1 antibody
Flotillin1 antibody
Integral membrane component of caveolae antibody
Reggie 2 antibody
图片
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Western blot analysis of Flotillin 1 on PC-3M cell lysate using anti-Flotillin 1 antibody at 1/500 dilution.
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Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin.
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Flow cytometric analysis of HUVEC cells with Flotillin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"