PPAR gamma Mouse Monoclonal Antibody [E-8]
Catalog# RT1668
PPAR gamma Mouse Monoclonal Antibody [E-8]
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WB
-
IHC-P
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IP
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Human
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Mouse
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Rat
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unconjugated
概述
产品名称
PPAR gamma Mouse Monoclonal Antibody [E-8]
抗体类型
Mouse Monoclonal Antibody
免疫原
specific for an epitope mapping between amino acids 486-505 at the C-terminus of PPARγ of human origin (identical to corresponding mouse sequence)
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IP
分子量
Predicted band size: 58 kDa
阳性对照
Jurkat cell lysate, U-937 cell lysate, THP-1 cell lysate, Mouse ovary tissue lysate, Mouse liver tissue lysate, Rat liver tissue lysate, mouse colon tissue, rat colon tissue.
偶联
unconjugated
克隆号
E-8
产品特性
形态
Liquid
浓度
存放说明
Store at 4℃.
存储缓冲液
1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
亚型
IgG1
应用稀释度
-
WB
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1:1,000
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IHC-P
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1:50-1:200
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IP
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1:1,000
靶点
功能
Peroxisome proliferator-activated receptors (PPARs), members of the nuclear hormone receptor subfamily of transcription factors, form heterodimers with retinoid X receptors (RXRs). These heterodimers regulate transcription of genes involved in Insulin action, adipocyte differentiation, lipid metabolism and inflammation. PPARg is implicated in diseases including obesity, diabetes, atherosclerosis and cancer. PPARg activators include prostanoids, fatty acids, thiazolidinediones and N-(2-benzoylphenyl) tyrosine analogues. PPARg is a key component in adipocyte differentiation and fat-specific gene expression. A Pro12Ala polymorphism of the PPARg2 gene may reduce transactivation activity in vitro. This substitution may affect the immune response to ox-LDL and be associated with type 2 diabetes. In addition, the Pro12Ala variant of the PPARg2 gene may be correlated with abdominal obesity in type 2 diabetes.
背景文献
1. Chen H et al. PPAR-gamma signaling in nonalcoholic fatty liver disease: Pathogenesis and therapeutic targets. Pharmacol Ther. 2023 May
2. Stark JM et al. The Role of PPAR-gamma in Allergic Disease. Curr Allergy Asthma Rep. 2021 Oct
亚细胞定位
Nucleus, Cytoplasm.
别名
CIMT1 antibody
GLM1 antibody
NR1C3 antibody
Nuclear receptor subfamily 1 group C member 3 antibody
OTTHUMP00000185032 antibody
OTTHUMP00000185036 antibody
Peroxisome proliferator activated nuclear receptor gamma variant 1 antibody
Peroxisome proliferator activated receptor gamma 1 antibody
Peroxisome Proliferator Activated Receptor gamma antibody
Peroxisome proliferator-activated receptor gamma antibody
展开图片
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Western blot analysis of PPAR gamma on different lysates with Mouse anti-PPAR gamma antibody (RT1668) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: U-937 cell lysate
Lane 3: THP-1 cell lysate
Lane 4: Mouse ovary tissue lysate
Lane 5: Mouse liver tissue lysate
Lane 6: Rat liver tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 58/50 kDa
Exposure time: 59 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (RT1668) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Mouse anti-PPAR gamma antibody (RT1668) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (RT1668) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Mouse anti-PPAR gamma antibody (RT1668) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (RT1668) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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A nutritional strategy for improving sub-adult grass carp (Ctenopharyngodon idella) flesh flavor: The role of α-lipoic acid in regulating muscle nucleotide and fatty acid metabolism
期刊: Aquaculture
DOI: 10.1016/j.aquaculture.2025.742437
IF: 3.9
应用: WB
反应种属: Fish
发表时间: 2025 Mar
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Nano SIM@ZIF-8 modified injectable High-intensity biohydrogel with bidirectional regulation of osteogenesis and Anti-adipogenesis for bone repair
期刊: Chemical Engineering Journal
DOI: 10.1016/j.cej.2022.134583
IF: 16.744
应用: WB,IHC-P
反应种属: Rat
发表时间: 2022 Jan
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