Human YKL-40/CHI3L1 DTSet enzyme-linked immunoassay kit
概述
产品名称
Human YKL-40/CHI3L1 DTSet enzyme-linked immunoassay kit
产品类型
DTSet Elisa Kit
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0851 | 0.0924 | 0.0888 | |
| 31.25 | 0.1902 | 0.2063 | 0.1983 | 0.1095 |
| 62.50 | 0.2916 | 0.2941 | 0.2929 | 0.2041 |
| 125.00 | 0.5265 | 0.4942 | 0.5104 | 0.4216 |
| 250.00 | 0.9138 | 0.8605 | 0.8872 | 0.7984 |
| 500.00 | 1.5760 | 1.5110 | 1.5435 | 1.4548 |
| 1000.00 | 2.7030 | 2.8010 | 2.7520 | 2.6633 |
| 2000.00 | 3.8440 | 3.8270 | 3.8355 | 3.7468 |
实验类型
夹心酶联免疫吸附测定(Sandwich Elisa)
种属反应性
Human
检测方法
比色法
实验检测时长
120min
标准曲线范围
31.25 pg/ml -2000 pg/ml
标准曲线梯度
7点/3倍
孵育次数
2
可检测样本
可溶性物质的液相样品。例如:血清,血浆,细胞培养上清,组织研磨液等。
加样体积
50 μl
产品特性
- 优化的捕获和检测抗体配对与推荐浓度可节省较长的开发时间
- 提供开发方案以指导进一步的分析优化
- 检测可根据您的特定需求进行定制
- 完整试剂盒的经济替代品
试剂盒组分
- 捕获抗体
- 检测抗体
- 重组标准品
- 与辣根过氧化物酶偶联的链霉亲和素 (Streptavidin-HRP)
所需的其他材料
DTSet辅助试剂盒(5板):包含96孔微孔板、封板膜、显色液、终止溶液、包板缓冲液(PBS)、洗涤缓冲液和检测缓冲液。
上面列出的部件可以单独购买:
- 96孔微孔板板(EP): YOUKE Life, Catalog # DSEP01
- 封板木膜(PS): YOUKE Life, Catalog # DSSF01。
- 包板缓冲液(CB): 137mM NaCl,2.7mM KCl,8.1mM Na2HPO4,1.5mM KH2PO4,pH 7.2-7.4,0.2m过滤。YOUKE Life, Catalog # DSCB01。
- 检测缓冲液 10X(AB): 0.5%BSA,0.05%Tween20,PBS溶液。YOUKE Life, Catalog # DSAB01。
- 封闭液(BB): YOUKE Life, Catalog # DSBB01。
- Washing Solution: 0.05%Tween20,PBS溶液,pH 7.2-7.4。YOUKE Life, Catalog # DSWB01。
- 底物显色液(TMB): TMB。YOUKE Life, Catalog # DSTS01。
- 终止溶液(SS): 0.5mol/ml H2SO4。YOUKE Life, Catalog # DSSS01。
靶点
功能
几丁质酶3样1(CHI3L1),在小鼠或人类中也称为brp39,是糖基水解酶18家族的39 kDa糖蛋白成员,尽管它没有显示壳三酶衍生酶活性。 CHI3L1由关节软骨细胞,滑膜细胞,活化的单核细胞衍生的巨噬细胞,中性粒细胞,内皮细胞,血管平滑肌细胞和某些癌细胞表达。 CHI3L1与几丁质和肝素结合,并增强细胞粘附,增殖和肿瘤血管生成。在炎症和结缔组织重塑期间,循环CHI3L1升高,例如关节炎,慢性阻塞性肺部疾病,糖尿病,心血管疾病,炎症性肠病和肝肝硬化。它经常在胶质母细胞瘤,粘液类软骨肉瘤,黑色素瘤和乳腺癌,甲状腺,结肠,肺,肾脏和卵巢中上调。
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