QKI-6 Recombinant Antibody [PSH10-23] - Rabbit IgG (Chimeric)
Catalog# HA723588
QKI-6 Recombinant Antibody [PSH10-23] - Rabbit IgG (Chimeric)
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IHC-Fr
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IHC-P
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WB
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Mouse
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Rat
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HA751497
不含抗保成分
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unconjugated
概述
产品名称
QKI-6 Recombinant Antibody [PSH10-23] - Rabbit IgG (Chimeric)
抗体类型
Recombinant Chimeric Antibody
免疫原
Synthetic peptide within human QKI-6 aa 281-319 / 319.
种属反应性
Mouse, Rat
验证应用
IHC-Fr, IHC-P, WB
分子量
Predicted band size: 35 kDa
阳性对照
Mouse cerebellum tissue, Rat cerebellum tissue, Mouse brain tissue lysate, Rat brain tissue lysate.
偶联
unconjugated
克隆号
PSH10-23
反应性数据
| IHC-Fr | IHC-P | WB | |
|---|---|---|---|
| Mouse |
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| Rat |
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产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
纯化方式
Protein A affinity purified.
应用稀释度
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IHC-Fr
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1:500
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IHC-P
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1:5,000
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WB
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1:5,000
靶点
功能
Quaking homolog, KH domain RNA binding (mouse), also known as QKI, is a protein which in humans is encoded by the QKI gene. QKI belongs to a family of RNA-binding proteins called STAR proteins for Signal Transduction and Activation of RNA. They have an HNRNPK homology (KH) domain embedded in a 200-amino acid region called the GSG domain. Other members of this family include SAM68 (KHDRBS1) and SF1. Two more new members are KHDRBS3 and KHDRBS2. The QKI gene is implicated as being important in schizophrenia, and QKI controls translation of many oligodendrocyte-related genes.
背景文献
1. Zhao Z et al. QKI shuttles internal m(7)G-modified transcripts into stress granules and modulates mRNA metabolism. Cell. 2023 Jul
2. Suh YY et al. MYB/MYBL1::QKI fusion-positive diffuse glioma. J Neuropathol Exp Neurol. 2023 Feb
亚细胞定位
Cytoplasm, cytosol, Nucleus.
别名
DKFZp586I0923 antibody
HKQ antibody
Homolog of mouse quaking QKI KH domain RNA binding protein antibody
Hqk antibody
HQK1 antibody
HqkI antibody
OTTHUMP00000017581 antibody
OTTHUMP00000017582 antibody
OTTHUMP00000017583 antibody
Protein quaking antibody
展开图片
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Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-QKI-6 antibody (HA723588) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723588) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of QKI-6 on different lysates with Rabbit anti-QKI-6 antibody (HA723588) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723588) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
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