TRAF1 Recombinant Rabbit Monoclonal Antibody [PSH13-88]
Catalog# HA723581
TRAF1 Recombinant Rabbit Monoclonal Antibody [PSH13-88]
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WB
-
IP
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Human
-
Rat
概述
产品名称
TRAF1 Recombinant Rabbit Monoclonal Antibody [PSH13-88]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human TRAF1 aa 1-200.
种属反应性
Human, Rat
验证应用
WB, IP
分子量
Predicted band size: 46 kDa
阳性对照
Raji cell lysate, Ramos cell lysate, Daudi cell lysate, Rat spleen tissue lysate.
偶联
unconjugated
克隆号
PSH13-88
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:10,000
-
IP
-
1-2μg/sample
靶点
功能
TNF receptor-associated factor 1 is a protein that in humans is encoded by the TRAF1 gene. The protein encoded by this gene is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from various receptors of the TNFR superfamily. This protein and TRAF2 form a heterodimeric complex, which is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF2 also interacts with IAP, and thus mediates the anti-apoptotic signals from TNF receptors. The expression of this protein can be induced by Epstein-Barr virus (EBV). EBV infection membrane protein 1 (LMP1) is found to interact with this and other TRAF proteins; this interaction is thought to link LMP1-mediated B lymphocyte transformation to the signal transduction from TNFR family receptors. TRAF1 also functions as a negative regulator of inflammation by interfering with the linear ubiquitination of NEMO downstream of TLR signaling. This explains why TRAF1 polymorphisms cause an increased risk for rheumatic diseases.
背景文献
1. Jang H et al. TRAF1 from a Structural Perspective. Biomolecules. 2024 Apr
2. Chen Y et al. N(6)-methyladenosine-modified TRAF1 promotes sunitinib resistance by regulating apoptosis and angiogenesis in a METTL14-dependent manner in renal cell carcinoma. Mol Cancer. 2022 May
亚细胞定位
Cytoplasm, cytosol.
别名
4732496E14Rik antibody
EBI6 antibody
EBV induced protein 6 antibody
Epstein-Barr virus-induced protein 6 antibody
MGC:10353 antibody
Tnf receptor associated factor 1 antibody
TNF receptor-associated factor 1 antibody
TRAF 1 antibody
TRAF1 antibody
TRAF1_HUMAN antibody
图片
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☑ Relative expression (RE)
Western blot analysis of TRAF1 on different lysates with Rabbit anti-TRAF1 antibody (HA723581) at 1/10,000 dilution.
Lane 1: Raji cell lysate (20 µg/Lane)
Lane 2: HEK-293 cell lysate (negative) (20 µg/Lane)
Lane 3: Ramos cell lysate (20 µg/Lane)
Lane 4: Daudi cell lysate (20 µg/Lane)
Lane 5: Rat spleen tissue lysate (40 µg/Lane)
Predicted band size: 46 kDa
Observed band size: 50 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723581) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
TRAF1 was immunoprecipitated from 0.2 mg Raji cell lysate with HA723581 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723581 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Raji cell lysate (input)
Lane 2: HA723581 IP in Raji cell lysate
Lane 3: Rabbit IgG instead of HA723581 in Raji cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 10 seconds; ECL: K1801
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Reactivity: Human,Rat
Conjugate: unconjugated
