BATF3 Recombinant Rabbit Monoclonal Antibody [PSH13-52]
Catalog# HA723541
BATF3 Recombinant Rabbit Monoclonal Antibody [PSH13-52]
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WB
-
IP
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ChIP
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Human
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HA751471
不含抗保成分
-
unconjugated
概述
产品名称
BATF3 Recombinant Rabbit Monoclonal Antibody [PSH13-52]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human BATF3 aa 1-127.
种属反应性
Human
验证应用
WB, IP, ChIP
分子量
Predicted band size: 14 kDa
阳性对照
U-937 cell lysate, HDLM2 cell lysate.
偶联
unconjugated
克隆号
PSH13-52
反应性数据
| WB | IP | ChIP | |
|---|---|---|---|
| Human |
|
|
|
| Mouse |
|
||
| Rat |
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:5,000
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IP
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1-2μg/sample
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ChIP
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Use 0.5~2 μg for 25 μg of chromatin.
靶点
功能
AP-1 family transcription factor that controls the differentiation of CD8+ thymic conventional dendritic cells in the immune system. Required for development of CD8-alpha+ classical dendritic cells (cDCs) and related CD103+ dendritic cells that cross-present antigens to CD8 T-cells and produce interleukin-12 (IL12) in response to pathogens (By similarity). Acts via the formation of a heterodimer with JUN family proteins that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3' and regulates expression of target genes.
背景文献
1. Jain N et al. TET2 guards against unchecked BATF3-induced CAR T cell expansion. Nature. 2023 Mar
2. Ziblat A et al. Batf3(+) DCs and the 4-1BB/4-1BBL axis are required at the effector phase in the tumor microenvironment for PD-1/PD-L1 blockade efficacy. Cell Rep. 2024 May
亚细胞定位
Nucleus.
UNIPROT
别名
21 kD small nuclear factor isolated from T cells antibody
21 kDa small nuclear factor isolated from T-cells antibody
ATF like 3 antibody
B-ATF-3 antibody
basic leucine zipper transcription factor, ATF-like 3 antibody
BATF3 antibody
FLJ36352 antibody
FLJ37535 antibody
hCG 39202 antibody
JDP1 antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of BATF3 on different lysates with Rabbit anti-BATF3 antibody (HA723541) at 1/5,000 dilution.
Lane 1: U-937 cell lysate
Lane 2: Ramos cell lysate (negative)
Lane 3: HDLM2 cell lysate
Lane 4: HepG2 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 14 kDa
Observed band size: 18 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723541) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
BATF3 was immunoprecipitated from 0.2 mg U-937 cell lysate with HA723541 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723541 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: U-937 cell lysate (input)
Lane 2: HA723541 IP in U-937 cell lysate
Lane 3: Rabbit IgG instead of HA723541 in U-937 cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 3 minutes; ECL: K1801 -
Chromatin immunoprecipitations were performed with cross-linked chromatin from U-937 cells with BATF3 (HA723541) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
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