Human FOLR1 Recombinant Rabbit Monoclonal Antibody [PSH11-59] - BSA and Azide free (Capture)
概述
产品名称
Human FOLR1 Recombinant Rabbit Monoclonal Antibody [PSH11-59] - BSA and Azide free (Capture)
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human FOLR1 aa 25-233.
种属反应性
Human
验证应用
ELISA(Cap)
阳性对照
Recombinant Human FOLR1 protein (HA211055).
偶联
unconjugated
克隆号
PSH11-59
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH11-60] to Human FOLR1 antibody (Detector) (HA723355) and Recombinant Human FOLR1 protein (HA211055) as the standard. The reference range value is 0.625-10 mg/mL.
靶点
功能
The protein encoded by this gene is a member of the folate receptor family. Members of this gene family bind folic acid and its reduced derivatives, and transport 5-methyltetrahydrofolate into cells. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. Mutations in this gene have been associated with neurodegeneration due to cerebral folate transport deficiency. Due to the presence of two promoters, multiple transcription start sites, and alternative splicing, multiple transcript variants encoding the same protein have been found for this gene. Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release. Required for normal embryonic development and normal cell proliferation.
背景文献
1. Wibowo A.S., Singh M., Reeder K.M., Carter J.J., Kovach A.R., Meng W., Ratnam M., Zhang F., Dann C.E. III. Structures of human folate receptors reveal biological trafficking states and diversity in folate and antifolate recognition. Proc. Natl. Acad. Sci. U.S.A. 110:15180-15188 (2013)
2. Rijnboutt S., Jansen G., Posthuma G., Hynes J.B., Schornagel J.H., Strous G.J. Endocytosis of GPI-linked membrane folate receptor-alpha. J. Cell Biol. 132:35-47 (1996)
亚细胞定位
Cell membrane. Cytoplasmic vesicle. Endosome. Membrane. Secreted.
UNIPROT
别名
adult antibody
Adult folate binding protein antibody
Adult folate-binding protein antibody
FBP antibody
Folate Binding Protein antibody
Folate Receptor 1 Adult antibody
Folate receptor 1 antibody
Folate Receptor 1 Precursor antibody
Folate receptor adult antibody
Folate receptor alpha antibody
展开图片
-
Sandwich ELISA analysis of human FOLR1 matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723354) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant human FOLR1 protein (HA211055) starting from 3,000 pg/ml to 0 pg/ml and detect antibody (HA723355, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
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