DNAJA1 Recombinant Rabbit Monoclonal Antibody [PSH07-73]
Catalog# HA722889
DNAJA1 Recombinant Rabbit Monoclonal Antibody [PSH07-73]
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WB
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IHC-P
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IF-Tissue
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IP
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Human
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Mouse
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Rat
概述
产品名称
DNAJA1 Recombinant Rabbit Monoclonal Antibody [PSH07-73]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human DNAJA1 aa 1-397.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IF-Tissue, IP
分子量
Predicted band size: 45 kDa
阳性对照
HepG2 cell lysate, HEK-293 cell lysate, Jurkat cell lysate, Raji cell lysate, SK-Br-3 cell lysate, A549 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, F9 cell lysate, PC-12 cell lysate, human kidney tissue, human testis tissue, mouse kidney tissue, mouse testis tissue, rat kidney tissue, rat testis tissue.
偶联
unconjugated
克隆号
PSH07-73
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:2,000
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IHC-P
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1:200-1:1,000
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IF-Tissue
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1:50-1:200
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IP
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1-2μg/sample
靶点
功能
This gene encodes a member of the DnaJ family of proteins, which act as heat shock protein 70 cochaperones. Heat shock proteins facilitate protein folding, trafficking, prevention of aggregation, and proteolytic degradation. Members of this family are characterized by a highly conserved N-terminal J domain, a glycine/phenylalanine-rich region, four CxxCxGxG zinc finger repeats, and a C-terminal substrate-binding domain. The J domain mediates the interaction with heat shock protein 70 to recruit substrates and regulate ATP hydrolysis activity. In humans, this gene has been implicated in positive regulation of virus replication through co-option by the influenza A virus. Several pseudogenes of this gene are found on other chromosomes.
背景文献
1. Kaida A et al. DNAJA1 promotes cancer metastasis through interaction with mutant p53. Oncogene. 2021 Aug
2. Roth HE et al. DNAJA1 Dysregulates Metabolism Promoting an Antiapoptotic Phenotype in Pancreatic Ductal Adenocarcinoma. J Proteome Res. 2021 Aug
亚细胞定位
Membrane, Cytoplasm, Microsome, Nucleus, Cytoplasm, perinuclear region, Mitochondrion.
别名
DJ 2 antibody
DJ2 antibody
DjA1 antibody
DnaJ (Hsp40) homolog, subfamily A, member 1 antibody
DnaJ homolog subfamily A member 1 antibody
DnaJ protein homolog 2 antibody
DNAJ2 antibody
Dnaja1 antibody
DNJA1_HUMAN antibody
hDJ 2 antibody
展开图片
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Western blot analysis of DNAJA1 on different lysates with Rabbit anti-DNAJA1 antibody (HA722889) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Raji cell lysate
Lane 5: SK-Br-3 cell lysate
Lane 6: A549 cell lysate
Lane 7: HeLa cell lysate
Lane 8: NIH/3T3 cell lysate
Lane 9: F9 cell lysate
Lane 10: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722889) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-DNAJA1 antibody (HA722889) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722889) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-DNAJA1 antibody (HA722889) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722889) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-DNAJA1 antibody (HA722889) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722889) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-DNAJA1 antibody (HA722889) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722889) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-DNAJA1 antibody (HA722889) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722889) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-DNAJA1 antibody (HA722889) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722889) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
DNAJA1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722889 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722889 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA722889 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA722889 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 6 seconds; ECL: K1801
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"