RNase L Recombinant Rabbit Monoclonal Antibody [JE36-11]
Catalog# HA722221
RNase L Recombinant Rabbit Monoclonal Antibody [JE36-11]
-
WB
-
Human
-
unconjugated
概述
产品名称
RNase L Recombinant Rabbit Monoclonal Antibody [JE36-11]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human RNase L aa 492-741 / 741.
种属反应性
Human
验证应用
WB
分子量
Predicted band size: 84 kDa
阳性对照
THP-1 cell lysate, THP-1 treated with 20ng/mL IFN-γ for 48 hours cell lysate, 293T cell lysate, HeLa cell lysate.
偶联
unconjugated
克隆号
JE36-11
反应性数据
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
靶点
功能
Ribonuclease L or RNase L (for latent), known sometimes as ribonuclease 4 or 2'-5' oligoadenylate synthetase-dependent ribonuclease, is an interferon (IFN)-induced ribonuclease which, upon activation, destroys all RNA within the cell (both cellular and viral). RNase L is an enzyme that in humans is encoded by the RNASEL gene. This gene encodes a component of the interferon-regulated 2'-5'oligoadenylate (2'-5'A) system that functions in the antiviral and antiproliferative roles of interferons. RNase L is activated by dimerization, which occurs upon 2'-5'A binding, and results in cleavage of all RNA in the cell. This can lead to activation of MDA5, an RNA helicase involved in the production of interferons.
背景文献
1. Boehmer DFR et al. OAS1/RNase L executes RIG-I ligand-dependent tumor cell apoptosis. Sci Immunol. 2021 Jul
2. Burke JM et al. RNase L activation in the cytoplasm induces aberrant processing of mRNAs in the nucleus. PLoS Pathog. 2022 Nov
亚细胞定位
Cytoplasm, Mitochondrion.
UNIPROT
别名
2 5A dependent ribonuclease antibody
2 5A dependent RNase antibody
2' 5' oligoisoadenylate synthetase dependent antibody
2-5A-dependent ribonuclease antibody
2-5A-dependent RNase antibody
DKFZp781D08126 antibody
Interferon induced 2 5A dependent RNase antibody
MGC104972 antibody
MGC133329 antibody
PRCA 1 antibody
展开图片
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☑ Cell treatment (CT)
Western blot analysis of RNase L on different lysates with Rabbit anti-RNase L antibody (HA722221) at 1/1,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: THP-1 treated with 20ng/mL IFN-γ for 48 hours cell lysate
Lane 3: 293T cell lysate
Lane 4: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 84 kDa
Observed band size: 75 kDa
Exposure time: Lane 1-2: 6 seconds; Lane 3-4: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722221) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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