Human CD200R1 Recombinant Rabbit Monoclonal Antibody [PSH05-02]
Catalog# HA722211
Human CD200R1 Recombinant Rabbit Monoclonal Antibody [PSH05-02]
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IF-Cell
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FC
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Human
概述
产品名称
Human CD200R1 Recombinant Rabbit Monoclonal Antibody [PSH05-02]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human CD200R1 aa 29-265 / 348.
种属反应性
Human
验证应用
IF-Cell, FC
分子量
Predicted band size: 39 kDa
阳性对照
Human peripheral blood.
偶联
unconjugated
克隆号
PSH05-02
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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IF-Cell
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1:100
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FC
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1:1,000
靶点
功能
Cell surface transmembrane glycoprotein CD200 receptor 1 is a protein that in humans is encoded by the CD200R1 gene. CD200R1 is expressed on the surface of myeloid cells and CD4+ T cells. It interacts with CD200 transmembrane glycoprotein that can be expressed on variety of cells including neurons, epithelial cells, endothelial cells, fibroblasts, and lymphoid cells. CD200R1 activation regulates the expression of pro-inflammatory molecules such as tumor necrosis factor (TNF-alpha), interferons, and inducible nitric oxide synthase (iNOS). This gene encodes a receptor for the OX-2 membrane glycoprotein. Both the receptor and substrate are cell surface glycoproteins containing two immunoglobulin-like domains. This receptor is restricted to the surfaces of myeloid lineage cells and the receptor-substrate interaction may function as a myeloid downregulatory signal. Mouse studies of a related gene suggest that this interaction may control myeloid function in a tissue-specific manner. Alternative splicing of this gene results in multiple transcript variants.
背景文献
1. Pannunzio B et al. CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury. Cells. 2022 May
2. Linley H et al. Reduced cutaneous CD200:CD200R1 signaling in psoriasis enhances neutrophil recruitment to skin. Immun Inflamm Dis. 2022 Jul
亚细胞定位
Cell membrane.
UNIPROT
别名
CD 200R antibody
CD200 cell surface glycoprotein receptor antibody
CD200 receptor 1 antibody
Cd200r1 antibody
Cell surface glycoprotein CD200 receptor 1 antibody
Cell surface glycoprotein OX2 receptor 1 antibody
Cell surface glycoprotein OX2 receptor antibody
cell surface glycoprotein receptor CD200 antibody
CRTR 2 antibody
CRTR2 antibody
展开图片
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☑ Cell treatment (CT)
Immunocytochemistry analysis of HeLa cells labeling Human CD200R1 with Rabbit anti-Human CD200R1 antibody (HA722211) at 1/100 dilution.
HeLa cells, transfected with empty control, CD200R1 or CD200R2 expression vector, respectively, were fixed in 4% paraformaldehyde for 10 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Human CD200R1 antibody (HA722211) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of human peripheral blood labeling Human CD200R1.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722211, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"