Ninein Recombinant Rabbit Monoclonal Antibody [PSH03-23]
Catalog# HA721965
Ninein Recombinant Rabbit Monoclonal Antibody [PSH03-23]
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WB
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Human
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Mouse
概述
产品名称
Ninein Recombinant Rabbit Monoclonal Antibody [PSH03-23]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human Ninein aa 1-500 / 2,090.
种属反应性
Human, Mouse
验证应用
WB
分子量
Predicted band size: 243 kDa
阳性对照
HeLa cell lysate, A549 cell lysate, HT-29 cell lysate, MEF cell lysate, human lung tissue lysate, mouse lung tissue lysate.
偶联
unconjugated
克隆号
PSH03-23
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:2,000
靶点
功能
Ninein, together with its paralog Ninein-like protein is one of the proteins important for centrosomal function. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells.
背景文献
1. He L et al. PTRN-1 (CAMSAP) and NOCA-2 (NINEIN) are required for microtubule polarity in Caenorhabditis elegans dendrites. PLoS Biol. 2022 Nov
2. Cao Z et al. β3-Endonexin interacts with ninein in vascular endothelial cells to promote angiogenesis. Biochem Biophys Res Commun. 2021 Aug
亚细胞定位
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole.
别名
Centrosome Marker antibody
Glycogen synthase kinase 3 beta interacting protein antibody
GSK3B interacting protein antibody
h Glycogen synthase kinase 3 beta-interacting protein antibody
hNinein antibody
KIAA1565 antibody
NIN antibody
NIN protein antibody
NIN_HUMAN antibody
ninein (GSK3B interacting protein) antibody
展开图片
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Western blot analysis of Ninein on different lysates with Rabbit anti-Ninein antibody (HA721965) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate
Lane 3: HT-29 cell lysate
Lane 4: MEF cell lysate
Lane 5: Human lung tissue lysate
Lane 6: Mouse lung tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 243 kDa
Observed band size: 270 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721965) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"