Phospho-MST1/MST2 (T180 + T183) Recombinant Rabbit Monoclonal Antibody [JE46-64]
Catalog# HA721737
Phospho-MST1/MST2 (T180 + T183) Recombinant Rabbit Monoclonal Antibody [JE46-64]
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WB
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Human
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Mouse
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Rat
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unconjugated
概述
产品名称
Phospho-MST1/MST2 (T180 + T183) Recombinant Rabbit Monoclonal Antibody [JE46-64]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic phosphopeptide corresponding to residues surrounding Thr183 of human MST1 protein.
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 56 kDa
阳性对照
Ramos treated with 1μM Staurosporine for 1 hour cell lysate, NIH/3T3 treated with 1μM Staurosporine for 3 hour cell lysate, C6 treated with 1μM Staurosporine for 3 hour cell lysate.
偶联
unconjugated
克隆号
JE46-64
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:1,000
靶点
功能
The human serine/threonine kinase, mammalian STE20 like kinase (MST), is considerably homologous to the budding yeast kinases, SPS1 and STE20, throughout their kinase domains. When stably expressed in HeLa cells, MST highly sensitizes the cells to death receptor mediated apoptosis by accelerating caspase 3 activation. These findings suggest that MST1 and MST2 play a role in apoptosis both upstream and downstream of caspase activation. MST1 is cleaved and activated by caspases during apoptosis and is capable of inducing apoptotic morphological changes such as chromatin condensation upon overexpression. Mammalian Sterile 20 Like 2 (MST2) is most similar to the previously identified MST1 protein kinase (78% identity, 88% similarity). Northern analysis indicates that MST2 mRNA is expressed at high levels in adult kidney, skeletal and placental tissues and at very low levels in adult heart, lung, liver and brain tissues. An in vitro kinase assay indicates that MST2 can phosphorylate an exogenous substrate, as well as itself, and phospho amino acid analysis indicates that it is a serine/threonine protein kinase.
背景文献
1. Liu M et al. Macrophage MST1/2 Disruption Impairs Post-Infarction Cardiac Repair via LTB4. Circ Res. 2021 Oct
2. Ueda Y et al. MST1/2 Balance Immune Activation and Tolerance by Orchestrating Adhesion, Transcription, and Organelle Dynamics in Lymphocytes. Front Immunol. 2020 May
亚细胞定位
Cytoplasm, Nucleus.
UNIPROT
别名
DKFZp686A2068 antibody
FLJ90748 antibody
KRS1 antibody
KRS2 antibody
Mammalian STE20 like protein kinase 1 antibody
Mammalian STE20 like protein kinase 2 antibody
MST 1 antibody
MST 2 antibody
MST1 antibody
MST2 antibody
展开图片
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☑ Cell treatment (CT)
Western blot analysis of Phospho-MST1/MST2 (T180 + T183) on different lysates with Rabbit anti-Phospho-MST1/MST2 (T180 + T183) antibody (HA721737) at 1/1,000 dilution.
Lane 1: Ramos cell lysate
Lane 2: Ramos treated with 1μM Staurosporine for 1 hour cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721737) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of Phospho-MST1/MST2 (T180 + T183) on different lysates with Rabbit anti-Phospho-MST1/MST2 (T180 + T183) antibody (HA721737) at 1/1,000 dilution.
Lane 1: NIH/3T3 cell lysate
Lane 2: NIH/3T3 treated with 1μM Staurosporine for 3 hour cell lysate
Lane 3: C6 cell lysate
Lane 4: C6 treated with 1μM Staurosporine for 3 hour cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 2 minutes 18 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721737) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Xuebijing Injection Alleviates Sepsis-Induced Acute Lung Injury by Inhibition of Cell Apoptosis and Inflammation Through the Hippo Pathway
期刊: Journal Of Inflammation Research
DOI: 10.2147/JIR.S523991
IF: 4.1
应用: WB
反应种属: Rat,Human
发表时间: 2025 Jun
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Cyclovirobuxine D Inhibits Triple-Negative Breast Cancer via YAP/TAZ Suppression and Activation of the FOXO3a/PINK1-Parkin Pathway-Induced Mitophagy
期刊: Phytomedicine
DOI:
IF: 6.7
应用: WB
反应种属: Human
发表时间: 2024 Nov
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