SNAP29 Recombinant Rabbit Monoclonal Antibody [PSH01-76]
Catalog# HA721728
SNAP29 Recombinant Rabbit Monoclonal Antibody [PSH01-76]
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WB
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Human
概述
产品名称
SNAP29 Recombinant Rabbit Monoclonal Antibody [PSH01-76]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human SNAP29 aa 1-258 / 258.
种属反应性
Human
验证应用
WB
分子量
Predicted band size: 29 kDa
阳性对照
HEK-293 cell lysate, HeLa cell lysate, Jurkat cell lysate, A549 cell lysate, U-87 MG cell lysate.
偶联
unconjugated
克隆号
PSH01-76
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
靶点
功能
SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. SNAP29 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. Also plays a role in ciliogenesis by regulating membrane fusions.
背景文献
1. Hou P, et al. 2023. The ORF7a protein of SARS-CoV-2 initiates autophagy and limits autophagosome-lysosome fusion via degradation of SNAP29 to promote virus replication.Autophagy. 19(2):551-569.
2. Zhou F, et al. 2018. Down-regulation of OGT promotes cisplatin resistance by inducing autophagy in ovarian cancer. Theranostics. 8(19):5200-5212.
亚细胞定位
Cytoplasm, Golgi apparatus membrane, Cytoplasmic vesicle, autophagosome membrane, Cell projection, cilium membrane.
UNIPROT
别名
CEDNIK antibody
FLJ21051 antibody
SNAP 29 antibody
SNAP-29 antibody
SNAP29 antibody
SNP29_HUMAN antibody
Soluble 29 kDa NSF attachment protein antibody
Synaptosomal associated protein 29 antibody
Synaptosomal associated protein 29kDa antibody
Synaptosomal-associated protein 29 antibody
展开图片
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Western blot analysis of SNAP29 on different lysates with Rabbit anti-SNAP29 antibody (HA721728) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: HeLa cell lysate
Lane 3: Jurkat cell lysate
Lane 4: A549 cell lysate
Lane 5: U-87 MG cell lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 5 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721728) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"