Human Interferon gamma Recombinant Rabbit Monoclonal Antibody [PSH01-45] - BSA and Azide free (Capture)
概述
产品名称
Human Interferon gamma Recombinant Rabbit Monoclonal Antibody [PSH01-45] - BSA and Azide free (Capture)
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human Interferon gamma aa 24-161.
种属反应性
Human
验证应用
ELISA(Cap)
阳性对照
Recombinant Human Interferon gamma protein (HA210762).
偶联
unconjugated
克隆号
PSH01-45
RRID
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH01-47] to Human Human Interferon gamma (Detector) (HA721685).
靶点
功能
Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
背景文献
1. Hisamatsu H., Shimbara N., Saito Y., Kristensen P., Hendil K.B., Fujiwara T., Takahashi E., Tanahashi N., Tamura T., Ichihara A., Tanaka K. Newly identified pair of proteasomal subunits regulated reciprocally by interferon gamma. J. Exp. Med. 183:1807-1816 (1996)
2. El Bougrini J., Pampin M., Chelbi-Alix M.K. Arsenic enhances the apoptosis induced by interferon gamma: key role of IRF-1. Cell. Mol. Biol. 52:9-15 (2006)
亚细胞定位
Secreted.
UNIPROT
别名
IF 1 antibody
IFG antibody
IFI antibody
ifn g antibody
IFN gamma antibody
IFN immune antibody
IFN, immune antibody
IFN-gamma antibody
IFNG antibody
IFNG_HUMAN antibody
展开图片
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![Standard curve of human IFNG matched pair antibodies:<br /><br />Capture: <a href="/products/HA721683" style="font-weight: bold;text-decoration: underline;">HA721683</a>, Interferon gamma Rabbit mAb [PSH01-45]<br />Detector: <a href="/products/HA721685" style="font-weight: bold;text-decoration: underline;">HA721685</a>, Interferon gamma Rabbit mAb [PSH01-47]<br /><br />Sandwich ELISA analysis of human IFNG matched pair antibodies<br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody [PSH01-45] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted IFNG protein starting from 2500 pg/ml to 0 pg/ml and detect antibody [PSH01-47]-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA721683_1.png?v=20250627160431)
Standard curve of human IFNG matched pair antibodies:
Capture: HA721683, Interferon gamma Rabbit mAb [PSH01-45]
Detector: HA721685, Interferon gamma Rabbit mAb [PSH01-47]
Sandwich ELISA analysis of human IFNG matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody [PSH01-45] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted IFNG protein starting from 2500 pg/ml to 0 pg/ml and detect antibody [PSH01-47]-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native IFN-gamma in human PBMC cell culture supernatant.<br /><br />Capture: <a href="/products/HA721683" style="font-weight: bold;text-decoration: underline;">HA721683</a>, Interferon gamma Rabbit mAb [PSH01-45]<br />Detector: <a href="/products/HA721685" style="font-weight: bold;text-decoration: underline;">HA721685</a>, Interferon gamma Rabbit mAb [PSH01-47]<br /><br />PBMC cells were stimulated with 10 µg/ml PHA-M or vehicle control and incubated for 48 hours. The concentrations of IFN-gamma were interpolated from the IFN-gamma standard curves and corrected for sample dilution. Undiluted samples are PHA-M stimulated PBMC supernatant 10% and unstimulated PBMC supernatant 10%. The mean IFN-gamma concentration was determined to be 12,543 pg/ml in PHA-M stimulated PBMC supernatant and undetectable in the unstimulated PBMC supernatant.](https://storage.huabio.cn/huabio/productImg/HA721683_2.png?v=20250627155259)
Interpolated concentrations of native IFN-gamma in human PBMC cell culture supernatant.
Capture: HA721683, Interferon gamma Rabbit mAb [PSH01-45]
Detector: HA721685, Interferon gamma Rabbit mAb [PSH01-47]
PBMC cells were stimulated with 10 µg/ml PHA-M or vehicle control and incubated for 48 hours. The concentrations of IFN-gamma were interpolated from the IFN-gamma standard curves and corrected for sample dilution. Undiluted samples are PHA-M stimulated PBMC supernatant 10% and unstimulated PBMC supernatant 10%. The mean IFN-gamma concentration was determined to be 12,543 pg/ml in PHA-M stimulated PBMC supernatant and undetectable in the unstimulated PBMC supernatant.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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