PCB Recombinant Rabbit Monoclonal Antibody [JE40-24]
Catalog# HA721546
PCB Recombinant Rabbit Monoclonal Antibody [JE40-24]
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WB
-
IHC-P
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Mouse
-
Rat
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Human
预测的种属支持售后
-
unconjugated
概述
产品名称
PCB Recombinant Rabbit Monoclonal Antibody [JE40-24]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human PCB aa 1050-1150.
种属反应性
Mouse, Rat (Predicted: Human)
预测的种属支持售后
验证应用
WB, IHC-P
分子量
Predicted band size: 130 kDa
阳性对照
Mouse liver tissue lysate, rat liver tissue lysate, mouse liver tissue.
偶联
unconjugated
克隆号
JE40-24
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
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IHC-P
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1:1,000
靶点
功能
Pyruvate carboxylase (PC) encoded by the gene PC is an enzyme (EC 6.4.1.1) of the ligase class that catalyzes (depending on the species) the physiologically irreversible carboxylation of pyruvate to form oxaloacetate (OAA). It is an important anaplerotic reaction that creates oxaloacetate from pyruvate. PC contains a biotin prosthetic group and is typically localized to the mitochondria in eukaryotes with exceptions to some fungal species such as Aspergillus nidulans which have a cytosolic PC. PC requires magnesium and zinc or manganese for catalysis. PC from different organisms exhibit varying degrees of activation by acetyl-CoA, but vertebrate PC typically requires it for activity.
背景文献
1. Kiesel VA et al. Pyruvate carboxylase and cancer progression. Cancer Metab. 2021 Apr
2. Schwörer S et al. Fibroblast pyruvate carboxylase is required for collagen production in the tumour microenvironment. Nat Metab. 2021 Nov
亚细胞定位
Mitochondrion matrix.
别名
mitochondrial antibody
OTTHUMP00000235155 antibody
OTTHUMP00000235156 antibody
PC antibody
PCB antibody
Pcx antibody
PYC_HUMAN antibody
Pyruvate carboxylase antibody
Pyruvate carboxylase mitochondrial antibody
Pyruvic carboxylase antibody
图片
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Western blot analysis of PCB on different lysates with Rabbit anti-PCB antibody (HA721546) at 1/1,000 dilution.
Lane 1: Mouse liver tissue lysate
Lane 2: Rat liver tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 130 kDa
Observed band size: 130 kDa
Exposure time: 1 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721546) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-PCB antibody (HA721546) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721546) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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