Rad50 Recombinant Rabbit Monoclonal Antibody [JE60-04]
Catalog# HA721492
Rad50 Recombinant Rabbit Monoclonal Antibody [JE60-04]
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WB
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Human
-
Mouse
-
Rat
-
unconjugated
概述
产品名称
Rad50 Recombinant Rabbit Monoclonal Antibody [JE60-04]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human Rad50 aa 1,162-1,312 / 1,312.
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 154 kDa
阳性对照
HeLa cell lysate, K-562 cell lysate, U-87 MG cell lysate, U-937 cell lysate, Caco-2 cell lysate, mouse testis tissue lysate, rat testis tissue lysate.
偶联
unconjugated
克隆号
JE60-04
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
靶点
功能
DNA repair protein RAD50, also known as RAD50, is a protein that in humans is encoded by the RAD50 gene. The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1 (also known as Xrs2 in yeast). This MRN complex (MRX complex in yeast) binds to broken DNA ends and displays numerous enzymatic activities that are required for double-strand break repair by nonhomologous end-joining or homologous recombination. Gene knockout studies of the mouse homolog of Rad50 suggest it is essential for cell growth and viability. Two alternatively spliced transcript variants of Rad50, which encode distinct proteins, have been reported.
背景文献
1. Kissling VM et al. Mre11-Rad50 oligomerization promotes DNA double-strand break repair. Nat Commun. 2022 May
2. Beikzadeh M et al. The dynamic nature of the Mre11-Rad50 DNA break repair complex. Prog Biophys Mol Biol. 2021 Aug
亚细胞定位
Nucleus, Chromosome, telomere.
别名
DNA repair protein RAD50 antibody
hRAD50 antibody
hsRAD50 antibody
Mrell antibody
NBSLD antibody
Rad 50 antibody
RAD50 2 antibody
rad50 antibody
RAD50 homolog (S cerevisiae) antibody
RAD50 homolog antibody
展开图片
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Western blot analysis of Rad50 on different lysates with Rabbit anti-Rad50 antibody (HA721492) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: U-87 MG cell lysate (20 µg/Lane)
Lane 4: U-937 cell lysate (20 µg/Lane)
Lane 5: Caco-2 cell lysate (20 µg/Lane)
Lane 6: Mouse testis tissue lysate (40 µg/Lane)
Lane 7: Rat testis tissue lysate (40 µg/Lane)
Predicted band size: 154 kDa
Observed band size: 154 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721492) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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