RbBP5 Recombinant Rabbit Monoclonal Antibody [PSH0-15]
Catalog# HA721273
RbBP5 Recombinant Rabbit Monoclonal Antibody [PSH0-15]
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WB
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Human
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Mouse
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Rat
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HA750585
不含抗保成分
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unconjugated
概述
产品名称
RbBP5 Recombinant Rabbit Monoclonal Antibody [PSH0-15]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human RbBP5 aa 431-480 / 538.
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 59 kDa
阳性对照
SW480 cell lysate, A431 cell lysate, C2C12 cell lysate, 293 cell lysate, MCF-7 cell lysate, NIH/3T3 cell lysate, K562 cell lysate, N2A cell lysate, PC-12 cell lysate, RAW264.7 cell lysate.
偶联
unconjugated
克隆号
PSH0-15
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:1,000
靶点
功能
Retinoblastoma-binding protein 5 is a protein that in humans is encoded by the RBBP5 gene. The protein encoded by this gene is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B.
背景文献
1. Xie H et al. Long non‑coding RNA AC245100.4 promotes prostate cancer tumorigenesis via the microRNA‑145‑5p/RBBP5 axis. Oncol Rep. 2021 Feb
2. Ali A et al. Diverse roles of WDR5-RbBP5-ASH2L-DPY30 (WRAD) complex in the functions of the SET1 histone methyltransferase family. J Biosci. 2017 Mar
亚细胞定位
Nucleus.
别名
RBBP 5 antibody
RBBP-5 antibody
RBBP5 antibody
RBBP5_HUMAN antibody
RBQ 3 antibody
RBQ3 antibody
Retinoblastoma binding protein 5 antibody
Retinoblastoma binding protein RBQ3 antibody
Retinoblastoma binding protein5 antibody
Retinoblastoma-binding protein 5 antibody
展开图片
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Western blot analysis of RbBP5 on different lysates with Rabbit anti-RbBP5 antibody (HA721273) at 1/1,000 dilution.
Lane 1: SW480 cell lysate
Lane 2: A431 cell lysate
Lane 3: C2C12 cell lysate
Lane 4: 293 cell lysate
Lane 5: MCF-7 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: K562 cell lysate
Lane 8: N2A cell lysate
Lane 9: PC-12 cell lysate
Lane 10: RAW264.7 cell lysate
Lysates/proteins at 25 µg/Lane.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721273) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of RbBP5 on different lysates with Rabbit anti-RbBP5 antibody (HA721273) at 1/1,000 dilution.
Lane 1: NIH-3T3-si NT cell lysate
Lane 2: NIH-3T3-si RbBP5 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Exposure time: 4 minutes;
4-20% SDS-PAGE gel.
HA721273 was shown to specifically react with RbBP5 in NIH-3T3-si NT cells. Weakened band was observed when NIH-3T3-si RbBP5 sample was tested. NIH-3T3-si NT and NIH-3T3-si RbBP5 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (HA721273, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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