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PEX11B Recombinant Rabbit Monoclonal Antibody [JE64-51]

RMB: 900 特惠 1500

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Catalog# HA721124

PEX11B Recombinant Rabbit Monoclonal Antibody [JE64-51]

Application

  • WB

Reactivity

  • Human

  • Mouse

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

PEX11B Recombinant Rabbit Monoclonal Antibody [JE64-51]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within human PEX11B aa 101-150/259.

种属反应性

Human, Mouse

验证应用

WB

分子量

Predicted band size: 28 kDa

阳性对照

Human lung tissue lysate, MCF-7 cell lysate.

偶联

unconjugated

克隆号

JE64-51

RRID

AB_3072248

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000

发表文章中的应用

WB 查看 1 篇文献如下

发表文章中的种属

Mouse 查看 1 篇文献如下
Rat 查看 1 篇文献如下

靶点

功能

The protein encoded by this gene facilitates peroxisomal proliferation and interacts with PEX19. The encoded protein is found in the peroxisomal membrane. Several transcript variants, some protein-coding and some not protein-coding, have been found for this gene.

背景文献

1. Tian Y. et. al. Variant analysis of PEX11B gene from a family with peroxisome biogenesis disorder 14B by whole exome sequencing. Mol Genet Genomic Med. 2020 Jan

2. Lismont C. et. al. Deciphering the potential involvement of PXMP2 and PEX11B in hydrogen peroxide permeation across the peroxisomal membrane reveals a role for PEX11B in protein sorting. Biochim Biophys Acta Biomembr. 2019 Oct

亚细胞定位

Peroxisome membrane.

UNIPROT

SwissProt: O96011 Human

别名

OTTHUMP00000015598 antibody

Peroxin 11B antibody

Peroxin-11B antibody

peroxisomal biogenesis factor 11 beta antibody

Peroxisomal biogenesis factor 11B antibody

Peroxisomal membrane protein 11B antibody

PEX11 beta antibody

PEX11-beta antibody

PEX11B antibody

Pex11p beta antibody

展开

图片

  • Western blot analysis of PEX11B on different lysates with Rabbit anti-PEX11B antibody (<a href="/products/HA721124" style="font-weight: bold;text-decoration: underline;">HA721124</a>) at 1/1,000 dilution.<br /><br />Lane 1: Human lung tissue lysate, 20 µg/Lane<br />Lane 2: MCF-7 cell lysate, 10 µg/Lane.<br /><br />Predicted band size: 28 kDa<br />Observed band size: 25 kDa<br /><br />Exposure time: 1 minute;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721124" style="font-weight: bold;text-decoration: underline;">HA721124</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PEX11B on different lysates with Rabbit anti-PEX11B antibody (HA721124) at 1/1,000 dilution.

    Lane 1: Human lung tissue lysate, 20 µg/Lane
    Lane 2: MCF-7 cell lysate, 10 µg/Lane.

    Predicted band size: 28 kDa
    Observed band size: 25 kDa

    Exposure time: 1 minute;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721124) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of PEX11B on different lysates with Rabbit anti-PEX11B antibody (<a href="/products/HA721124" style="font-weight: bold;text-decoration: underline;">HA721124</a>) at 1/1,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-PEX11B KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 28 kDa<br />Observed band size: 28 kDa<br /><br />Exposure time: 40 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721124" style="font-weight: bold;text-decoration: underline;">HA721124</a>) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of PEX11B on different lysates with Rabbit anti-PEX11B antibody (HA721124) at 1/1,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-PEX11B KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 28 kDa
    Observed band size: 28 kDa

    Exposure time: 40 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721124) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
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