Oncostatin M Recombinant Rabbit Monoclonal Antibody [JE40-04]
Catalog# HA721038
Oncostatin M Recombinant Rabbit Monoclonal Antibody [JE40-04]
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WB
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IHC-P
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Human
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Mouse
概述
产品名称
Oncostatin M Recombinant Rabbit Monoclonal Antibody [JE40-04]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human Oncostatin M aa 26-150.
种属反应性
Human, Mouse
验证应用
WB, IHC-P
分子量
Predicted band size: 28 kDa
阳性对照
A549 cell lysate, MCF-7 cell lysate, Hela cell lysate, mouse kidney tissue lysate, human colon carcinoma tissue.
偶联
unconjugated
克隆号
JE40-04
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500
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IHC-P
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1:100
靶点
功能
This gene encodes a member of the leukemia inhibitory factor/oncostatin-M (LIF/OSM) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein is a secreted cytokine and growth regulator that inhibits the proliferation of a number of tumor cell lines. This protein also regulates the production of other cytokines, including interleukin 6, granulocyte-colony stimulating factor and granulocyte-macrophage colony stimulating factor in endothelial cells. This gene and the related gene, leukemia inhibitory factor, also present on chromosome 22, may have resulted from the duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
背景文献
1. Hermanns HM. Oncostatin M and interleukin-31: Cytokines, receptors, signal transduction and physiology. Cytokine Growth Factor Rev. 2015 Oct;26(5):545-58.
2. West NR, et al. Oncostatin M drives intestinal inflammation and predicts response to tumor necrosis factor-neutralizing therapy in patients with inflammatory bowel disease. Nat Med. 2017 May;23(5):579-589.
亚细胞定位
Secreted.
别名
MGC20461 antibody
ONCM_HUMAN antibody
Oncostatin M antibody
Oncostatin-M antibody
OSM antibody
图片
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Western blot analysis of Oncostatin M on different lysates with Rabbit anti-Oncostatin M antibody (HA721038) at 1/500 dilution.
Lane 1: A549 cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: Hela cell lysate
Lane 4: Mouse kidney tissue lysate (20 µg/Lane)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 22 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721038) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Oncostatin M antibody (HA721038) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721038) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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