iFluor™ 647 Conjugated Cytokeratin 6 Recombinant Rabbit Monoclonal Antibody [SN71-07]
Catalog# HA720143F
iFluor™ 647 Conjugated Cytokeratin 6 Recombinant Rabbit Monoclonal Antibody [SN71-07]
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IF-Cell
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IF-Tissue
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Human
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Rat
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iFluor™ 647
概述
产品名称
iFluor™ 647 Conjugated Cytokeratin 6 Recombinant Rabbit Monoclonal Antibody [SN71-07]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human Cytokeratin 6 aa 1-118 / 564.
种属反应性
Human, Rat
验证应用
IF-Cell, IF-Tissue
分子量
Predicted band size: 60 kDa
阳性对照
A431, human tonsil tissue.
偶联
iFluor™ 647
克隆号
SN71-07
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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IF-Cell
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1:100
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IF-Tissue
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1:100
靶点
功能
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The alpha-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins, which are useful markers of tissue differentiation, also aid in the characterization of malignant tumors. IL-1 and TNFα induce transcription of cytokeratin 6 in epidermal keratinocytes via the C/EBP β transcription factor. In humans, multiple isoforms of cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies.
背景文献
1. Kubo A., et al. 2013. Collapse of the keratin filament network through the expression of mutant keratin 6c observed in a case of focal plantar keratoderma. J. Dermatol. 40:553-557.
2. Akasaka E., et al. 2011. Diffuse and focal palmoplantar keratoderma can be caused by a keratin 6c mutation. Br. J. Dermatol. 165:1290-1292.
UNIPROT
别名
CK6 antibody
CK 6A antibody
CK 6B antibody
CK 6C antibody
CK 6D antibody
CK 6E antibody
CK-6B antibody
CK-6C antibody
CK-6E antibody
Cytokeratin 6a antibody
展开图片
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Immunocytochemistry analysis of A431 cells labeling Cytokeratin 6 with Rabbit anti-Cytokeratin 6 antibody (HA720143F) at 1/100 dilution.
Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37℃. Cells were then incubated with Rabbit anti-Cytokeratin 6 antibody (HA720143F, red) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, green) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/800 dilution. -
Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling Cytokeratin 6 (HA720143F) and Vimentin (EM0401).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 6 (HA720143F, red) at 1/100 dilution and Vimentin (EM0401, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.
iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.
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