iFluor™ 488 Conjugated Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody [ST05-43]
Catalog# HA720134F
iFluor™ 488 Conjugated Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody [ST05-43]
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IF-Tissue
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Human
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Rat
概述
产品名称
iFluor™ 488 Conjugated Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody [ST05-43]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Cytokeratin 10 aa 541-584 / 584.
种属反应性
Human, Rat
验证应用
IF-Tissue
分子量
Predicted band size: 59 kDa
阳性对照
Human skin tissue, rat skin tissue.
偶联
iFluor™ 488
克隆号
ST05-43
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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IF-Tissue
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1:400-1:500
靶点
功能
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. Cytokeratins 10 and 13 are present in the cytoskeletal region of a subset of squamous cell carcinomas. Cytokeratin 10 is a heterotetramer of two type I and two type II keratins, is generally associated with keratin 1, and is seen in all suprabasal cell layers including stratum corneum.
背景文献
1. Tabuchi Y. et al. Development of oral epithelial cell line ROE2 with differentiation potential from transgenic rats harboring temperature-sensitive simian virus40 large T-antigen gene. Exp Anim 63:31-44 (2014).
2. Zhu XJ. et al. BMP-FGF signaling axis mediates Wnt-induced epidermal stratification in developing mammalian skin. PLoS Genet 10:e1004687 (2014).
亚细胞定位
Cytoplasm.
别名
BCIE antibody
BIE antibody
CK 10 antibody
CK10 antibody
CK-10 antibody
Cytokeratin-10 antibody
EHK antibody
K10 antibody
K1C10_HUMAN antibody
Keratin 10 antibody
展开图片
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Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 10 (HA720134F) and Vimentin (EM0401).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (HA720134F, green) at 1/500 dilution and Vimentin (EM0401, red) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.
iFluor™ 594 conjugate-Goat anti-Mouse IgG (HA1126) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain. -
Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 10 (HA720134F), Cytokeratin 14 (HA720147F) and Vimentin (EM0401).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (HA720134F, green) at 1/400 dilution, Cytokeratin 14 (HA720147F, red) at 1/400 dilution and Vimentin (EM0401, yellow) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.
Alexa Fluor® 555 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"