p75 NGF Receptor Recombinant Rat Monoclonal Antibody [PSH13-26] - BSA and Azide free
Catalog# HA610274
p75 NGF Receptor Recombinant Rat Monoclonal Antibody [PSH13-26] - BSA and Azide free
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IHC-Fr
-
WB
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Mouse
-
Rat
概述
产品名称
p75 NGF Receptor Recombinant Rat Monoclonal Antibody [PSH13-26] - BSA and Azide free
抗体类型
Recombinant Rat Monoclonal Antibody
种属反应性
Mouse, Rat
验证应用
IHC-Fr, WB
分子量
Predicted band size: 46 kDa
阳性对照
Mouse brain tissue lysate, Rat brain tissue lysate.
偶联
unconjugated
克隆号
PSH13-26
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
IHC-Fr
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1:200
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WB
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1:2,000
靶点
功能
The p75 neurotrophin receptor (p75NTR) was first identified in 1973 as the low-affinity nerve growth factor receptor (LNGFR) before discovery that p75NTR bound other neurotrophins equally well as nerve growth factor. p75NTR is a neurotrophic factor receptor. Neurotrophic factor receptors bind Neurotrophins including Nerve growth factor, Neurotrophin-3, Brain-derived neurotrophic factor, and Neurotrophin-4. All neurotrophins bind to p75NTR. This also includes the immature pro-neurotrophin forms. Neurotrophic factor receptors, including p75NTR, are responsible for ensuring a proper density to target ratio of developing neurons, refining broader maps in development into precise connections. p75NTR is involved in pathways that promote neuronal survival and neuronal death.
背景文献
1. Bruno F et al. The Nerve Growth Factor Receptor (NGFR/p75(NTR)): A Major Player in Alzheimer\'s Disease. Int J Mol Sci. 2023 Feb
2. Castellini C et al. The Effect of Interaction NGF/p75(NTR) in Sperm Cells: A Rabbit Model. Cells. 2022 Mar
亚细胞定位
Cell membrane, Cytoplasm, Perikaryon, Cell projection, growth cone, dendritic spine.
别名
CD271 antibody
CD271 antigen antibody
Gp80 LNGFR antibody
Gp80-LNGFR antibody
Low affinity nerve growth factor receptor antibody
Low affinity neurotrophin receptor p75NTR antibody
Low-affinity nerve growth factor receptor antibody
Nerve growth factor receptor antibody
Nerve growth factor receptor TNFR superfamily member 16 antibody
NGF receptor antibody
展开图片
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Application: IHC-Fr
Species: Mouse
Site: brain (medial septum)
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Mouse
Site: colon
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Not required -
Western blot analysis of p75 NGF Receptor on different lysates with Rat anti-p75 NGF Receptor antibody (HA610274) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 46 kDa
Observed band size: 70 kDa
Exposure time: 59 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610274) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"