Cytokeratin 17 Recombinant Mouse Monoclonal Antibody [A2B10-R] - BSA and Azide free
Catalog# HA610110
Cytokeratin 17 Recombinant Mouse Monoclonal Antibody [A2B10-R] - BSA and Azide free
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WB
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IF-Cell
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IHC-P
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Human
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HA601235
含抗保成分
-
unconjugated
概述
产品名称
Cytokeratin 17 Recombinant Mouse Monoclonal Antibody [A2B10-R] - BSA and Azide free
抗体类型
Recombinant Mouse Monoclonal Antibody
免疫原
Synthetic peptide within Human Cytokeratin 17 aa 383-432 / 432.
种属反应性
Human
验证应用
WB, IF-Cell, IHC-P
分子量
Predicted band size: 48 kDa
阳性对照
HeLa cell lysate, A431 cell lysate, MCF7 cell lysate, HepG2 cell lysate, HeLa, human cervical cancer tissue, human lung adenocarcinoma tissue.
偶联
unconjugated
克隆号
A2B10-R
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:1,000
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IF-Cell
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1:100
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IHC-P
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1:1,000
靶点
功能
Type I keratin involved in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair (By similarity). Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state (By similarity). Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway (By similarity). Involved in tissue repair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells". Acts as a promoter of epithelial proliferation by acting a regulator of immune response in skin: promotes Th1/Th17-dominated immune environment contributing to the development of basaloid skin tumors (By similarity). May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation.
背景文献
1. Shen Z. et. al. HLA DR B1*04, *07-restricted epitopes on Keratin 17 for autoreactive T cells in psoriasis. J. Dermatol. Sci. 38:25-39(2005).
2. Shen Z. et. al. Altered keratin 17 peptide ligands inhibit in vitro proliferation of keratinocytes and T cells isolated from patients with psoriasis. J. Am. Acad. Dermatol. 54:992-1002(2006).
亚细胞定位
Cytoplasm.
UNIPROT
别名
39.1 antibody
CK 17 antibody
CK17 antibody
CK-17 antibody
Cytokeratin-17 antibody
K17 antibody
K1C17_HUMAN antibody
Keratin 17 antibody
keratin 17 epitope S1 antibody
keratin 17 epitope S2 antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of Cytokeratin 17 on different lysates with Mouse anti-Cytokeratin 17 antibody (HA610110) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: MCF7 cell lysate (negative)
Lane 4: HepG2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 5 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610110) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling Cytokeratin 17 with Mouse anti-Cytokeratin 17 antibody (HA610110) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Cytokeratin 17 antibody (HA610110) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue with Mouse anti-Cytokeratin 17 antibody (HA610110) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610110) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue with Mouse anti-Cytokeratin 17 antibody (HA610110) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610110) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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