AKR1C1 Recombinant Mouse Monoclonal Antibody [5-F10-R]
Catalog# HA601174
AKR1C1 Recombinant Mouse Monoclonal Antibody [5-F10-R]
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WB
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IF-Cell
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Human
概述
产品名称
AKR1C1 Recombinant Mouse Monoclonal Antibody [5-F10-R]
抗体类型
Recombinant Mouse Monoclonal Antibody
免疫原
Recombinant protein within Human AKR1C1 aa 180-323 / 323.
种属反应性
Human
验证应用
WB, IF-Cell
分子量
Predicted band size: 36.8 kDa
阳性对照
HepG2 cell lysate, U-87 MG cell lysate, HeLa cell lysate, A431 cell lysate, human liver tissue lysate, HeLa.
偶联
unconjugated
克隆号
5-F10-R
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
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IF-Cell
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1:100
靶点
功能
Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentrations of NADPH. Displays a broad positional specificity acting on positions 3, 17 and 20 of steroids and regulates the metabolism of hormones like estrogens and androgens. May also reduce conjugated steroids such as 5alpha-dihydrotestosterone sulfate. Displays affinity for bile acids.
背景文献
1. Woo S et al. AKR1C1 as a Biomarker for Differentiating the Biological Effects of Combustible from Non-Combustible Tobacco Products. Genes (Basel) 8(5) (2017).
2. Zeng CM et al. Aldo-Keto Reductase AKR1C1-AKR1C4: Functions, Regulation, and Intervention for Anti-cancer Therapy. Front Pharmacol 8:119 (2017).
亚细胞定位
Cytoplasm.
UNIPROT
别名
2-dihydrobenzene-1 antibody
2-diol dehydrogenase antibody
20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody
20 ALPHA HSD antibody
20 alpha hydroxysteroid dehydrogenase antibody
20-alpha-HSD antibody
20-alpha-hydroxysteroid dehydrogenase antibody
20ALPHAHSD antibody
2ALPHAHSD antibody
AK1C1 antibody
展开图片
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Western blot analysis of AKR1C1 on different lysates with Mouse anti-AKR1C1 antibody (HA601174) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: U-87 MG cell lysate (20 µg/Lane)
Lane 3: HeLa cell lysate (20 µg/Lane)
Lane 4: A431 cell lysate (20 µg/Lane)
Lane 5: Human liver tissue lysate (40 µg/Lane)
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601174) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling AKR1C1 with Mouse anti-AKR1C1 antibody (HA601174) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-AKR1C1 antibody (HA601174) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"