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Collagen IV Rabbit Polyclonal Antibody

RMB: 900 特惠 1500

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Catalog# HA500197

Collagen IV Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Collagen IV Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

Recombinant protein within C-terminal human Collagen IV.

种属反应性

Human

验证应用

WB, IF-Cell

分子量

161 kDa

阳性对照

MCF-7 cell lysate, SH-SY5Y cell lysate, SH-SY5Y.

偶联

unconjugated

RRID

AB_3071294

产品特性

形态

Liquid

浓度

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:50-1:200

靶点

功能

This gene encodes a type IV collagen alpha protein. Type IV collagen proteins are integral components of basement membranes. This gene shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in this gene cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants.

背景文献

1. Itai T. et. al. Prenatal clinical manifestations in individuals with COL4A1/2 variants. J Med Genet. 2020 Jul

2. Wang T. et. al. COL4A1 promotes the growth and metastasis of hepatocellular carcinoma cells by activating FAK-Src signaling. J Exp Clin Cancer Res. 2020 Aug

亚细胞定位

Basement membrane.

UNIPROT

SwissProt: P02462 Human

别名

Collagen alpha-1(IV) chain

Arresten

COL4A1

Collagen IV

图片

  • Western blot analysis of Collagen IV on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/HA500197" style="font-weight: bold;text-decoration: underline;">HA500197</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: MCF-7 cell lysate<br />Lane 2: SH-SY5Y cell lysate

    Western blot analysis of Collagen IV on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500197, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: MCF-7 cell lysate
    Lane 2: SH-SY5Y cell lysate

  • ICC staining of Collagen IV in SH-SY5Y cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/HA500197" style="font-weight: bold;text-decoration: underline;">HA500197</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Collagen IV in SH-SY5Y cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500197, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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