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Goat Anti-Mouse IgG polyclonal Antibody

RMB: 200

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Catalog# HA1013

Goat Anti-Mouse IgG polyclonal Antibody

Application

  • WB

  • ELISA

Reactivity

  • Mouse

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Goat Anti-Mouse IgG polyclonal Antibody

抗体类型

Goat Polyclonal Antibody

免疫原

Mouse IgG.

产品特异性

To minimize cross-reactivity, the goat anti-mouse IgG (H+L) antibodies have been highly cross-adsorbed against human IgG.

种属反应性

Mouse

验证应用

WB, ELISA

阳性对照

Mouse IgG lysate.

偶联

unconjugated

产品特性

形态

Liquid

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

PBS (pH7.4).

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • WB

  • 1:5000

  • ELISA

  • 1:5000

发表文章中的应用

WB 查看 4 篇文献如下

发表文章中的种属

None 查看 1 篇文献如下
Mouse 查看 1 篇文献如下

靶点

功能

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

背景文献

暂无

图片

  • Western blot analysis of Goat Anti-Rabbit IgG on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/HA1013" style="font-weight: bold;text-decoration: underline;">HA1013</a>, 1/5000) was used in 5% BSA at room temperature for 2 hours. Rabbit Anti-Goat IgG - HRP Secondary Antibody (<a href="/products/HA1005" style="font-weight: bold;text-decoration: underline;">HA1005</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br />Positive control:<br />Lane 1: Mouse IgG lysate<br />Lane 2: Rabbit IgG cell lysate

    Western blot analysis of Goat Anti-Rabbit IgG on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA1013, 1/5000) was used in 5% BSA at room temperature for 2 hours. Rabbit Anti-Goat IgG - HRP Secondary Antibody (HA1005) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Mouse IgG lysate
    Lane 2: Rabbit IgG cell lysate

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
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