AP-A Recombinant Rabbit Monoclonal Antibody [JE46-03]
Catalog# ET7109-45
AP-A Recombinant Rabbit Monoclonal Antibody [JE46-03]
-
WB
-
IHC-P
-
Human
-
unconjugated
概述
产品名称
AP-A Recombinant Rabbit Monoclonal Antibody [JE46-03]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human AP-A aa 860-957 / 957.
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 109 kDa
阳性对照
Human kidney tissue lysate, human small intestine tissue lysate, human kidney tissue, human small intestine tissue.
偶联
unconjugated
克隆号
JE46-03
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:500-1:2,000
-
IHC-P
-
1:200-1:1,000
靶点
功能
Regulates central hypertension through its calcium-modulated preference to cleave N-terminal acidic residues from peptides such as angiotensin II. ENPEP is considered to be an essential and highly specific enzyme, which metabolizes and inactivates bioactive peptides such as angiotensin II (AngII) in the renin-angiotensin system. ENPEP is expressed in neoplastic lesions of the uterine cervix, and its levels are upregulated as the lesions progress from cervical intraepithelial neoplasms to invasive squamous cell carcinomas. ENPEP expression is absent in normal cells and is overexpressed in cancer cell lines compared with normal cells of mammary origin.
背景文献
1. Yang Y et al. Structural insights into central hypertension regulation by human aminopeptidase A. J Biol Chem 288:25638-25645 (2013).
2. Ino K et al. Expression of aminopeptidase A in human gestational choriocarcinoma cell lines and tissues. Placenta 21:63-72 (2000).
序列相似性
Belongs to the peptidase M1 family.
组织特异性
Expressed in choriocarcinoma cancer cell lines (at protein level). Expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. Also found in a variety of other tissues.
亚细胞定位
Cell membrane, Membrane.
UNIPROT
别名
Aminopeptidase A antibody
AMPE_HUMAN antibody
AP-A antibody
APA antibody
bp-1 antibody
bp1 antibody
CD249 antibody
Differentiation antigen gp160 antibody
EAP antibody
Enpep antibody
展开图片
-
Western blot analysis of AP-A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-45, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human kidney tissue lysate
Lane 2: Human small intestine tissue lysate -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-AP-A antibody (ET7109-45) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-45) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-AP-A antibody (ET7109-45) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-45) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
浙公网安备 33019202000643号