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NOXA2 Recombinant Rabbit Monoclonal Antibody [JG86-31]

RMB: 900 特惠 1500

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Catalog# ET7108-72

NOXA2 Recombinant Rabbit Monoclonal Antibody [JG86-31]

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

概述

产品名称

NOXA2 Recombinant Rabbit Monoclonal Antibody [JG86-31]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Recombinant protein within Human NOXA2 aa 180-320 / 526.

种属反应性

Human, Mouse, Rat

验证应用

WB, IHC-P, IP

分子量

60/47 kDa

阳性对照

Rat spleen tissue lysates, rat spleen tissue, human spleen tissue, mouse spleen tissue.

偶联

unconjugated

克隆号

JG86-31

RRID

AB_3070867

产品特性

形态

Liquid

浓度

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:10-1:50

靶点

功能

The heredity disease chronic granulomatous disease (CGF) has been linked to mutations in p47phox and p67phox. The cytosolic proteins p47phox and p67phox, also designated neutrophil cytosol factor 1(NCF1) and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47phox and p67phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47phox and p67phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 domain of p67phox has been shown to interact with the proline rich domain of p47phox, suggesting that p47phox may faciliate the transport of p67phox to the membrane.

背景文献

1. Yoshida L S et al. Expression of a p67(phox) homolog in Caco-2 cells giving O(2)(-)-reconstituting ability to cytochrome b(558) together with recombinant p47(phox). Biochem Biophys Res Commun 296:1322-1328 (2002).

2. de Boer M et al. Autosomal recessive chronic granulomatous disease with absence of the 67-kD cytosolic NADPH oxidase component: identification of mutation and detection of carriers. Blood 83:531-536 (1994).

序列相似性

Belongs to the NCF2/NOXA1 family.

亚细胞定位

Cytoplasm.

UNIPROT

SwissProt: P19878 Human

SwissProt: O70145 Mouse

SwissProt: A7E3N2 Rat

别名

67 kDa neutrophil oxidase factor antibody

Chronic granulomatous disease autosomal 2 antibody

FLJ93058 antibody

NADPH oxidase activator 2 antibody

NCF-2 antibody

Ncf2 antibody

NCF2_HUMAN antibody

Neutrophil cytosol factor 2 antibody

Neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2) antibody

Neutrophil NADPH oxidase factor 2 antibody

展开

图片

  • Western blot analysis of NOXA2 on rat spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET7108-72" style="font-weight: bold;text-decoration: underline;">ET7108-72</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:40,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of NOXA2 on rat spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-72, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-NOXA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-72" style="font-weight: bold;text-decoration: underline;">ET7108-72</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-NOXA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-72, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NOXA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-72" style="font-weight: bold;text-decoration: underline;">ET7108-72</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NOXA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-72, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-NOXA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-72" style="font-weight: bold;text-decoration: underline;">ET7108-72</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-NOXA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-72, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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