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Gephyrin Recombinant Rabbit Monoclonal Antibody [JG34-47]

RMB: 900 特惠 1500

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Catalog# ET7108-04

Gephyrin Recombinant Rabbit Monoclonal Antibody [JG34-47]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Gephyrin Recombinant Rabbit Monoclonal Antibody [JG34-47]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Recombinant protein within Human Gephyrin aa 5-95 / 736.

种属反应性

Human, Mouse, Rat

验证应用

WB, IHC-P

分子量

Predicted band size: 80 kDa

阳性对照

SK-Br-3 cell lysate, Neuro-2a cell lysate, C6 cell lysate, mouse brain tissue lysate, mouse liver tissue lysate, mouse kidney tissue lysate, human kidney tissue, mouse brain tissue.

偶联

unconjugated

克隆号

JG34-47

RRID

AB_3070807

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000

  • IHC-P

  • 1:200-1:400

发表文章中的应用

IF 查看 1 篇文献如下

发表文章中的种属

Marmoset 查看 1 篇文献如下

靶点

功能

The sub-membraneous region at the postsynaptic membrane contains a number of proteins critical for receptor targeting. Gephyrin is a microtubule-associated protein highly expressed in brain and localized to neuronal postsynaptic membranes. Gephyrin is essential for the postsynaptic localization of the inhibitory glycine receptor and is thought to anchor the receptor to subsynaptic microtubules. The protein is expressed in most mammalian tissues with predominant expression in brain. At least five additional splice variants of Gephyrin ranging in molecular weight have been identified in rat and human brain tissue.

背景文献

1. Dejanovic B et al. Simultaneous impairment of neuronal and metabolic function of mutated gephyrin in a patient with epileptic encephalopathy. EMBO Mol Med 7:1580-1594 (2015).

2. Reiss J et al. A GPHN point mutation leading to molybdenum cofactor deficiency. Clin Genet 80:598-599 (2011).

序列相似性

In the N-terminal section; belongs to the MoaB/Mog family.; In the C-terminal section; belongs to the MoeA family.

亚细胞定位

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Postsynaptic cell membrane, Synapse.

UNIPROT

SwissProt: Q9NQX3 Human

SwissProt: Q8BUV3 Mouse

SwissProt: Q03555 Rat

别名

Domain E antibody

Domain G antibody

GEPH antibody

GEPH_HUMAN antibody

GPH antibody

GPHN antibody

GPHRYN antibody

KIAA1385 antibody

Molybdopterin molybdenumtransferase antibody

MPT adenylyltransferase antibody

展开

图片

  • Western blot analysis of Gephyrin on different lysates with Rabbit anti-Gephyrin antibody (<a href="/products/ET7108-04" style="font-weight: bold;text-decoration: underline;">ET7108-04</a>) at 1/1,000 dilution.<br /><br />Lane 1: SK-Br-3 cell lysate (15 µg/Lane)<br />Lane 2: Neuro-2a cell lysate (15 µg/Lane)<br />Lane 3: C6 cell lysate (15 µg/Lane)<br />Lane 4: Mouse brain tissue lysate (30 µg/Lane)<br />Lane 5: Mouse liver tissue lysate (30 µg/Lane)<br />Lane 6: Mouse kidney tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 80 kDa<br />Observed band size: 80 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7108-04" style="font-weight: bold;text-decoration: underline;">ET7108-04</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Gephyrin on different lysates with Rabbit anti-Gephyrin antibody (ET7108-04) at 1/1,000 dilution.

    Lane 1: SK-Br-3 cell lysate (15 µg/Lane)
    Lane 2: Neuro-2a cell lysate (15 µg/Lane)
    Lane 3: C6 cell lysate (15 µg/Lane)
    Lane 4: Mouse brain tissue lysate (30 µg/Lane)
    Lane 5: Mouse liver tissue lysate (30 µg/Lane)
    Lane 6: Mouse kidney tissue lysate (30 µg/Lane)

    Predicted band size: 80 kDa
    Observed band size: 80 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-04) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-Gephyrin antibody (<a href="/products/ET7108-04" style="font-weight: bold;text-decoration: underline;">ET7108-04</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-04" style="font-weight: bold;text-decoration: underline;">ET7108-04</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-Gephyrin antibody (ET7108-04) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-04) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rabbit anti-Gephyrin antibody (<a href="/products/ET7108-04" style="font-weight: bold;text-decoration: underline;">ET7108-04</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-04" style="font-weight: bold;text-decoration: underline;">ET7108-04</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rabbit anti-Gephyrin antibody (ET7108-04) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-04) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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