Integrin alpha 4 Recombinant Rabbit Monoclonal Antibody [JA09-36]
Catalog# ET1704-31
Integrin alpha 4 Recombinant Rabbit Monoclonal Antibody [JA09-36]
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WB
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IHC-P
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FC
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Human
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Mouse
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Rat
概述
产品名称
Integrin alpha 4 Recombinant Rabbit Monoclonal Antibody [JA09-36]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Integrin alpha 4 aa 983-1032 / 1032.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, FC
分子量
Predicted band size: 115 kDa
阳性对照
Jurkat cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, RAW264.7 cell lysate, human tonsil tissue, human spleen tissue, Jurkat.
偶联
unconjugated
克隆号
JA09-36
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
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IHC-P
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1:50-1:200
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FC
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1:10-1:50
靶点
功能
Integrins are heterodimers composed of noncovalently associated transmembrane α and β subunits. The 16 α and 8 β subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including fibronectin, collagen and vitronectin. Certain integrins can also bind to soluble ligands such as fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors; both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis.
背景文献
1. Totsuka N et al. Toll-like receptor 4 and MAIR-II/CLM-4/LMIR2 immunoreceptor regulate VLA-4-mediated inflammatory monocyte migration. Nat Commun 5:4710 (2014).
2. Qi J et al. Identification, characterization, and epitope mapping of human monoclonal antibody J19 that specifically recognizes activated integrin a4 7. J Biol Chem 287:15749-59 (2012).
序列相似性
Belongs to the LAMP family.
组织特异性
Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is detected in spleen, thymus, prostate, testis, small intestine, colon, skeletal muscle, brain, placenta, lung, kidney, ovary and pancreas and liver. Isoform LAMP-2C is detected in small intestine, colon, heart, brain, skeletal muscle, and at lower levels in kidney and placenta.
翻译后修饰
O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.
亚细胞定位
Membrane.
别名
269C wild type antibody
Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
CD49 antigen like family member D antibody
CD49 antigen-like family member D antibody
CD49d antibody
IA4 antibody
Integrin alpha 4 antibody
Integrin alpha 4 subunit antibody
Integrin alpha IV antibody
Integrin alpha-4 antibody
展开图片
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Western blot analysis of Integrin alpha 4 on different lysates with Rabbit anti-Integrin alpha 4 antibody (ET1704-31) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: Mouse spleen tissue lysate (40 µg/Lane)
Lane 3: Rat spleen tissue lysate (40 µg/Lane)
Predicted band size: 115 kDa
Observed band size: 70/140/150 kDa
Exposure time: Lane 1: 25 seconds; Lane 2-3: 5 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-31) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of Integrin alpha 4 on different lysates with Rabbit anti-Integrin alpha 4 antibody (ET1704-31) at 1/1,000 dilution.
Lane 1: RAW264.7 cell lysate
Lane 2: RAW264.7 cell lysate treated with deglycosylation
Lysates/proteins at 20 µg/Lane.
Predicted band size: 115 kDa
Observed band size: 70/140/150 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-31) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Integrin alpha 4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-31, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Integrin alpha 4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-31, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of Integrin alpha 4 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-31, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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