Hsp75 Recombinant Rabbit Monoclonal Antibody [SD08-71]
Catalog# ET1612-54
Hsp75 Recombinant Rabbit Monoclonal Antibody [SD08-71]
-
WB
-
IP
-
Human
概述
产品名称
Hsp75 Recombinant Rabbit Monoclonal Antibody [SD08-71]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human Hsp75 aa 560-704 / 704.
种属反应性
Human
验证应用
WB, IP
分子量
75 kDa
阳性对照
Hela cell lysate, 293T cell lysate.
偶联
unconjugated
克隆号
SD08-71
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000-1:2,000
靶点
功能
The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent polypeptide chains across cellular membranes and regulation of protein folding. Heat shock protein 75 mitochondrial precursor (HSP 75), also called tumor necrosis factor type 1 receptor-associated protein (TRAP1), is a 704 amino acid member of the heat shock protein 90 family. HSP 75 localizes to the mitochondrion and is expressed in a variety of tissues, including skeletal muscle, liver, heart, brain, pancreas, lung and placenta, functioning as a chaperone that expresses an ATPase activity.
背景文献
1. Condelli, V. et al. 2015. Targeting TRAP1 as a downstream effector of BRAF cytoprotective pathway: a novel strategy for human BRAF-driven colorectal carcinoma. Oncotarget. 6: 22298-309.
2. Campos-Martorell, M. et al. 2014. Brain proteomics identifies potential simvastatin targets in acute phase of stroke in a rat embolic model. Journal of neurochemistry. 130: 301-12.
序列相似性
Belongs to the heat shock protein 90 family.
组织特异性
Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung, placenta and bladder. Expression is highly reduced in bladder cancer and renal cell carcinoma specimens compared to healthy tissues, but it is increased in other type of tumors.
亚细胞定位
Mitochondrion, Mitochondrion inner membrane, Mitochondrion matrix.
UNIPROT
别名
Heat Shock Protein 75 antibody
Heat shock protein 75 kDa antibody
Heat shock protein 75 kDa mitochondrial antibody
HSP 75 antibody
HSP90L antibody
mitochondrial antibody
TNF receptor associated protein 1 antibody
TNFR Associated Protein antibody
TNFR-associated protein 1 antibody
TRAP-1 antibody
展开图片
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Western blot analysis of Hsp75 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-54, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: 293T cell lysate
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"