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Integrin alpha E Recombinant Rabbit Monoclonal Antibody [SN62-06]

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Catalog# ET1611-27

Integrin alpha E Recombinant Rabbit Monoclonal Antibody [SN62-06]

Application

  • IHC-P

Reactivity

  • Human

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Integrin alpha E Recombinant Rabbit Monoclonal Antibody [SN62-06]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human Integrin alpha E aa 1-50 / 1,179.

种属反应性

Human

验证应用

IHC-P

分子量

Predicted band size: 130 kD

阳性对照

Human tonsil tissue, human lymph node tissue, human small intestine tissue.

偶联

unconjugated

克隆号

SN62-06

RRID

AB_3070004

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • IHC-P

  • 1:50-1:1000

发表文章中的应用

mIHC 查看 1 篇文献如下

发表文章中的种属

Human 查看 1 篇文献如下

靶点

功能

Integrin, alpha E (ITGAE) also known as CD103 (cluster of differentiation 103) is an integrin protein that in human is encoded by the ITGAE gene. CD103 binds integrin beta 7 (β7– ITGB7) to form the complete heterodimeric integrin molecule αEβ7, which has no distinct name. The αEβ7 complex is often referred to as "CD103" though this strictly refers only to the αE chain. Note that the β7 subunit can bind with other integrin α chains, such as α4 (CD49d). The chief ligand for αEβ7 is E-cadherin, a cellular adhesion molecule (CAM) found on epithelial cells. It is probably important for T cell homing to the intestinal sites and thymocyte contacts with thymic reticuloepithelial cells. Tregs are important for decreasing the immune response and appear to play a crucial role in the prevention of autoimmune diseases. Tregs are defined as CD4+/CD25+/Foxp3+ cells. Some CD4+/FoxP3− cells also express CD103 and have been attributed regulatory activity.

背景文献

1. Webb JR et al. Tumor-infiltrating lymphocytes expressing the tissue resident memory marker CD103 are associated with increased survival in high-grade serous ovarian cancer. Clin Cancer Res 20:434-44 (2014).

2. Morgan EA et al. Immunohistochemical detection of hairy cell leukemia in paraffin sections using a highly effective CD103 rabbit monoclonal antibody. Am J Clin Pathol 139:220-30 (2013).

序列相似性

Belongs to the integrin alpha chain family.

组织特异性

Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells.

亚细胞定位

Membrane.

UNIPROT

SwissProt: P38570 Human

别名

A530055J10 antibody

alpha-E1 antibody

alpha-M290 antibody

aM290 antibody

Antigen CD103 antibody

CD 103 antibody

CD103 antibody

CD103 antigen antibody

HML 1 antigen antibody

HML-1 antigen antibody

展开

图片

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Integrin alpha E antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1611-27" style="font-weight: bold;text-decoration: underline;">ET1611-27</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Integrin alpha E antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lymph node tissue using anti-Integrin alpha E antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1611-27" style="font-weight: bold;text-decoration: underline;">ET1611-27</a>, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lymph node tissue using anti-Integrin alpha E antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-27, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Integrin alpha E antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1611-27" style="font-weight: bold;text-decoration: underline;">ET1611-27</a>, 1/1000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Integrin alpha E antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-27, 1/1000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. mIHC
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