概述
产品名称
PARP1 Recombinant Rabbit Monoclonal Antibody [SU03-68]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within N-terminal human PARP1.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IF-Tissue, IHC-P, FC
分子量
Predicted band size: 113 kDa
阳性对照
A549 cell lysate, Jurkat cell lysate, Hela cell lysate, HeLa, human spleen tissue, mouse large intestine tissue, human breast carcinoma tissue, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate.
偶联
unconjugated
克隆号
SU03-68
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
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IF-Cell
-
1:500
-
IF-Tissue
-
1:50-1:800
-
IHC-P
-
1:500-1:4,000
-
FC
-
1:1,000
发表文章中的应用
靶点
功能
The main role of PARP (found in the cell nucleus) is to detect and initiate an immediate cellular response to metabolic, chemical, or radiation-induced single-strand DNA breaks (SSB) by signaling the enzymatic machinery involved in the SSB repair. Once PARP detects a SSB, it binds to the DNA, undergoes a structural change, and begins the synthesis of a polymeric adenosine diphosphate ribose (poly (ADP-ribose) or PAR) chain, which acts as a signal for the other DNA-repairing enzymes. Target enzymes include DNA ligase III (LigIII), DNA polymerase beta (polβ), and scaffolding proteins such as X-ray cross-complementing gene 1 (XRCC1). After repairing, the PAR chains are degraded via Poly(ADP-ribose) glycohydrolase (PARG). PARP enzymes are essential in a number of cellular functions, including expression of inflammatory genes: PARP1 is required for the induction of ICAM-1 gene expression by cardiac myocytes and smooth muscle cells, in response to TNF.
背景文献
1. Cao C et al. The long intergenic noncoding RNA UFC1, a target of MicroRNA 34a, interacts with the mRNA stabilizing protein HuR to increase levels of -catenin in HCC cells. Gastroenterology 148:415-26.e18 (2015).
2. Gao S et al. Ischemia-reperfusion injury of the retina is linked to necroptosis via the ERK1/2-RIP3 pathway. Mol Vis 20:1374-87 (2014).
翻译后修饰
Phosphorylated by PRKDC and TXK.; Poly-ADP-ribosylated on glutamate and aspartate residues by autocatalysis. Poly-ADP-ribosylated by PARP2; poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites. ADP-ribosylated on serine by autocatalysis; serine ADP-ribosylation takes place following interaction with HPF1.; S-nitrosylated, leading to inhibit transcription regulation activity.
亚细胞定位
Nucleus, nucleolus, chromosome.
UNIPROT #
别名
ADP ribosyltransferase (NAD+
poly (ADP ribose) polymerase) antibody
ADP ribosyltransferase diphtheria toxin like 1 antibody
ADP ribosyltransferase NAD(+) antibody
ADPRT 1 antibody
ADPRT antibody
ADPRT1 antibody
ARTD1 antibody
msPARP antibody
NAD(+) ADP ribosyltransferase 1 antibody
展开ADP ribosyltransferase (NAD+
poly (ADP ribose) polymerase) antibody
ADP ribosyltransferase diphtheria toxin like 1 antibody
ADP ribosyltransferase NAD(+) antibody
ADPRT 1 antibody
ADPRT antibody
ADPRT1 antibody
ARTD1 antibody
msPARP antibody
NAD(+) ADP ribosyltransferase 1 antibody
NAD(+) ADP-ribosyltransferase 1 antibody
pADPRT 1 antibody
pADPRT1 antibody
PARP 1 antibody
PARP antibody
PARP-1 antibody
PARP1 antibody
PARP1_HUMAN antibody
Poly (ADP ribose) polymerase 1 antibody
poly (ADP ribose) polymerase family, member 1 antibody
Poly [ADP-ribose] polymerase 1 antibody
Poly(ADP ribose) polymerase antibody
poly(ADP ribose) synthetase antibody
poly(ADP ribosyl)transferase antibody
Poly[ADP ribose] synthetase 1 antibody
Poly[ADP-ribose] synthase 1 antibody
PPOL antibody
折叠图片
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Western blot analysis of PARP1 on different lysates with Rabbit anti-PARP1 antibody (ET1608-56) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: Jurkat cell lysate (15 µg/Lane)
Lane 3: NIH/3T3 cell lysate (15 µg/Lane)
Lane 4: C2C12 cell lysate (15 µg/Lane)
Lane 5: C6 cell lysate (15 µg/Lane)
Lane 6: PC-12 cell lysate (15 µg/Lane)
Predicted band size: 113 kDa
Observed band size: 113 kDa
Exposure time: 6 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-56) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of PARP1 on different lysates with Rabbit anti-PARP1 antibody (ET1608-56) at 1/2,000 dilution.
Lane 1: HeLa whole cell lysate
Lane 2: HeLa treated with 1μM staurosporine for 3 hours whole cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 113 kDa
Observed band size: 113/28 kDa
Exposure time: 1 minute 9 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-56) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. -
All lanes: Western blot analysis of PARP with anti-PARP1 antibody[SU03-68] (ET1608-56) at 1:500 dilution.
Lane 1/2: Wild-type Hela whole cell lysate (10 µg).
Lane 3/4: PARP knockdown Hela whole cell lysate (10 µg).
ET1608-56 was shown to specifically react with PARP in wild-type Hela cells. Weakened bands were observed when PARP knockdown samples were tested. Wild-type and PARP knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1608-56, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling PARP1 with Rabbit anti-PARP1 antibody (ET1608-56) at 1/500 dilution.
Cells were fixed in 100% methanol for 5 minutes, blocked with 2% normal goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PARP1 antibody (ET1608-56) at 1/500 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, green) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/800 dilution. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-PARP1 antibody (ET1608-56) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-56) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-PARP1 antibody (ET1608-56) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-56) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of HeLa cells labeling PARP1.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1608-56, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-PARP1 antibody (ET1608-56) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-56) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-PARP1 antibody (ET1608-56) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-56) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-PARP1 antibody (ET1608-56) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-56) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-PARP1 antibody (ET1608-56) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-56) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-PARP1 antibody (ET1608-56) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-56) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Apatinib Inhibits Bladder Cancer through Suppression of the VEGFR2-PI3K-AKT Signaling Pathway as Revealed by Network Pharmacology and in vitro Experimental Verification
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Fangchinoline exerts anticancer effects on colorectal cancer by inducing autophagy via regulation AMPK/mTOR/ULK1 pathway. Biochemical pharmacology, 186, 114475.
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