Active Caspase-3 Recombinant Rabbit Monoclonal Antibody [SR01-02]
概述
产品名称
Active Caspase-3 Recombinant Rabbit Monoclonal Antibody [SR01-02]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Caspase-3 aa 28-67 / 277.
种属反应性
Human, Pig
验证应用
WB, IF-Cell, IF-Tissue, IHC-P
分子量
Predicted band size: 17 kDa
阳性对照
Camptothecin (2 μM) treated Jurkat cell lysate, Hela, PC-3M, human colon carcinoma tissue, human placenta tissue.
偶联
unconjugated
克隆号
SR01-02
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000-1:2,000
-
IF-Cell
-
1:50-1:200
-
IF-Tissue
-
1:50-1:200
-
IHC-P
-
1:50-1:200
发表文章中的应用
靶点
功能
Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9. It is encoded by the CASP3 gene. CASP3 orthologs have been identified in numerous mammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts. Caspase-3 shares many of the typical characteristics common to all currently-known caspases. For example, its active site contains a cysteine residue (Cys-163) and histidine residue (His-121) that stabilize the peptide bond cleavage of a protein sequence to the carboxy-terminal side of an aspartic acid when it is part of a particular 4-amino acid sequence. This specificity allows caspases to be incredibly selective, with a 20,000-fold preference for aspartic acid over glutamic acid. A key feature of caspases in the cell is that they are present as zymogens, termed procaspases, which are inactive until a biochemical change causes their activation. Each procaspase has an N-terminal large subunit of about 20 kDa followed by a smaller subunit of about 10 kDa, called p20 and p10, respectively.
背景文献
1. Xu TX et al. Hypoxia responsive miR-210 promotes cell survival and autophagy of endometriotic cells in hypoxia. Eur Rev Med Pharmacol Sci 20:399-406 (2016).
2. Huang X et al. Dose-dependent inhibitory effects of zoledronic acid on osteoblast viability and function in vitro. Mol Med Rep 13:613-22 (2016).
序列相似性
Belongs to the peptidase C14A family.
组织特异性
Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
翻译后修饰
Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.; S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
亚细胞定位
Cytoplasm
UNIPROT #
别名
Caspase3
A830040C14Rik antibody
Apopain antibody
CASP 3 antibody
CASP-3 antibody
CASP3 antibody
CASP3_HUMAN antibody
Casp3a antibody
Caspase 3 antibody
Caspase 3, apoptosis-related cysteine peptidase antibody
展开Caspase3
A830040C14Rik antibody
Apopain antibody
CASP 3 antibody
CASP-3 antibody
CASP3 antibody
CASP3_HUMAN antibody
Casp3a antibody
Caspase 3 antibody
Caspase 3, apoptosis-related cysteine peptidase antibody
Caspase 3, apoptosis-related cysteine protease antibody
Caspase 3, apoptosis-related cysteine protease a antibody
Caspase-3 subunit p12 antibody
Caspase3 antibody
CC3 antibody
CPP 32 antibody
CPP-32 antibody
CPP32 antibody
CPP32B antibody
Cysteine protease CPP32 antibody
EC 3.4.22.56 antibody
ICE3 antibody
LICE antibody
mldy antibody
OTTHUMP00000165052 antibody
OTTHUMP00000165053 antibody
OTTHUMP00000165054 antibody
PARP cleavage protease antibody
Procaspase3 antibody
Protein Yama antibody
SCA 1 antibody
SCA-1 antibody
SCA1 antibody
SREBP cleavage activity 1 antibody
Yama antibody
Yama protein antibody
折叠图片
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Western blot analysis of Active Caspase-3 on different lysates with Rabbit anti-Active Caspase-3 antibody (ET1602-47) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: Jurkat treated with 25μM Etoposide for 5 hours cell lysate
Lane 3: Jurkat treated with 25μM Etoposide for 16 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-47) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Active Caspase-3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-47, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Camptothecin (2 μM) treated Jurkat cell lysate
Lane 2: Untreated Jurkat cell lysate -
ICC staining of Active Caspase-3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-47, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of Active Caspase-3 in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-47, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Active Caspase-3 antibody (ET1602-47) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-47) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Active Caspase-3 antibody (ET1602-47) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-47) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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