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Alkaline Phosphatase Recombinant Rabbit Monoclonal Antibody [SA40-00]

RMB: 900 特惠 1500

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Catalog# ET1601-21

Alkaline Phosphatase Recombinant Rabbit Monoclonal Antibody [SA40-00]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Alkaline Phosphatase Recombinant Rabbit Monoclonal Antibody [SA40-00]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within human Alkaline Phosphatase aa 18-50.

种属反应性

Human, Mouse, Rat

验证应用

WB, IHC-P, IF-Tissue

分子量

Predicted band size: 57 kDa

阳性对照

Saos-2 cell lysate, HeLa cell lysate, A549 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, HepG2, SW480, mouse kidney tissue, mouse jawbone tissue, rat liver tissue, mouse liver tissue, Hela.

偶联

unconjugated

克隆号

SA40-00

RRID

AB_3069604

产品特性

形态

Liquid

浓度

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:5,000

  • IHC-P

  • 1:1,000-1:8,000

  • IF-Tissue

  • 1:200

靶点

功能

This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects.

背景文献

1. Chen M. et. al. Low-dose X-ray irradiation promotes osteoblast proliferation, differentiation and fracture healing. PLoS One 9:e104016 (2014).

序列相似性

Belongs to the alkaline phosphatase family.

翻译后修饰

N-glycosylated.

亚细胞定位

Cell membrane, Mitochondrion membrane, Mitochondrion intermembrane space, Extracellular vesicle membrane.

UNIPROT

SwissProt: P05186 Human

SwissProt: P09242 Mouse

SwissProt: P08289 Rat

别名

Alkaline phosphatase antibody

Alkaline phosphatase placental antibody

Alkaline phosphatase placental type antibody

Alkaline phosphatase Regan isozyme antibody

ALP antibody

Alp1 antibody

ALPP antibody

FLJ61142 antibody

Germ-cell alkaline phosphatase antibody

nagao isozyme antibody

展开

图片

  • Western blot analysis of Alkaline Phosphatase on different lysates with Rabbit anti-Alkaline Phosphatase antibody (<a href="/products/ET1601-21" style="font-weight: bold;text-decoration: underline;">ET1601-21</a>) at 1/5,000 dilution.<br /><br />Lane 1: Saos-2 cell lysate (15 µg/Lane)<br />Lane 2: HeLa cell lysate (15 µg/Lane)<br />Lane 3: A549 cell lysate (15 µg/Lane)<br />Lane 4: Mouse liver tissue lysate (20 µg/Lane)<br />Lane 5: Rat liver tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 57 kDa<br />Observed band size: 75 kDa<br />Exposure time: 24 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1601-21" style="font-weight: bold;text-decoration: underline;">ET1601-21</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Alkaline Phosphatase on different lysates with Rabbit anti-Alkaline Phosphatase antibody (ET1601-21) at 1/5,000 dilution.

    Lane 1: Saos-2 cell lysate (15 µg/Lane)
    Lane 2: HeLa cell lysate (15 µg/Lane)
    Lane 3: A549 cell lysate (15 µg/Lane)
    Lane 4: Mouse liver tissue lysate (20 µg/Lane)
    Lane 5: Rat liver tissue lysate (20 µg/Lane)

    Predicted band size: 57 kDa
    Observed band size: 75 kDa
    Exposure time: 24 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-21) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Alkaline Phosphatase antibody (<a href="/products/ET1601-21" style="font-weight: bold;text-decoration: underline;">ET1601-21</a>) at 1/8,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1601-21" style="font-weight: bold;text-decoration: underline;">ET1601-21</a>) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Alkaline Phosphatase antibody (ET1601-21) at 1/8,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-21) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Alkaline Phosphatase antibody (<a href="/products/ET1601-21" style="font-weight: bold;text-decoration: underline;">ET1601-21</a>) at 1/8,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1601-21" style="font-weight: bold;text-decoration: underline;">ET1601-21</a>) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Alkaline Phosphatase antibody (ET1601-21) at 1/8,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-21) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Alkaline Phosphatase antibody (<a href="/products/ET1601-21" style="font-weight: bold;text-decoration: underline;">ET1601-21</a>) at 1/8,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1601-21" style="font-weight: bold;text-decoration: underline;">ET1601-21</a>) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Alkaline Phosphatase antibody (ET1601-21) at 1/8,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-21) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse jawbone tissue with Rabbit anti-Alkaline Phosphatase antibody (<a href="/products/ET1601-21" style="font-weight: bold;text-decoration: underline;">ET1601-21</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1601-21" style="font-weight: bold;text-decoration: underline;">ET1601-21</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse jawbone tissue with Rabbit anti-Alkaline Phosphatase antibody (ET1601-21) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-21) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: liver <br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/200

    Application: IF-Tissue

    Species: Human

    Site: liver

    Sample: Paraffin-embedded section

    Antibody concentration: 1/200

  • Application: IF-Tissue<br /><br />Species: Mouse<br /><br />Site: bone<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/200

    Application: IF-Tissue

    Species: Mouse

    Site: bone

    Sample: Paraffin-embedded section

    Antibody concentration: 1/200

  • Application: IF-Tissue<br /><br />Species: Mouse<br /><br />Site: bone<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/200

    Application: IF-Tissue

    Species: Mouse

    Site: bone

    Sample: Paraffin-embedded section

    Antibody concentration: 1/200

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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