This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
iFluor™ 488 Conjugated Anti-Collagen VI Recombinant Rabbit Monoclonal Antibody
Predicted band size: 109 kDa
Recombinant protein within Human Collagen VI aa 17-255 / 1,028.
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Protein A affinity purified.
SwissProt: P12109 Human
SwissProt: P12110 Human
SwissProt: P12111 Human
SwissProt: Q02788 Mouse
SwissProt: Q04857 Mouse
- Alpha 1 (VI) chain (61 AA) antibody
- CO6A1_HUMAN antibody
- COL6A1 antibody
- COL6A2 antibody
- COL6A3 antibody
- Collagen alpha 2(VI) chain antibody
- Collagen alpha 3(VI) chain antibody
- Collagen alpha-1(VI) chain antibody
- Collagen type VI alpha 1 antibody
- Collagen type VI alpha 2 antibody
- Collagen type VI alpha 3 antibody
- Collagen VI alpha 1 polypeptide antibody
- Collagen VI alpha 2 polypeptide antibody
- Collagen VI alpha 3 polypeptide antibody
- CollagenVI antibody
- Human mRNA for collagen VI alpha 2 C terminal globular domain antibody
- OPLL antibody
- PP3610 antibody
Fig1: Immunocytochemistry analysis of Hela cells labeling Collagen VI with Rabbit anti-Collagen VI antibody (HA720155F) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-Collagen VI antibody (HA720155F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.
Fig2: Immunofluorescence analysis of paraffin-embedded mouse colon tissue labeling Collagen VI (HA720155F).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Collagen VI (HA720155F, iFluor™ 488) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.
1. Kristofik N et al. Impaired von Willebrand factor adhesion and platelet response in thrombospondin-2 knockout mice. Blood 128:1642-50 (2016).
2. Okawa S et al. Lipopolysaccharide induces expression of collagen VI in the rat lung. J Toxicol Pathol 28:37-41 (2015).
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