iFluor™ 488 Conjugated Collagen VI Recombinant Rabbit Monoclonal Antibody [SD83-03]
Catalog# HA720155F
iFluor™ 488 Conjugated Collagen VI Recombinant Rabbit Monoclonal Antibody [SD83-03]
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IF-Cell
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IF-Tissue
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Human
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Mouse
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Rat
概述
产品名称
iFluor™ 488 Conjugated Collagen VI Recombinant Rabbit Monoclonal Antibody [SD83-03]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human Collagen VI aa 17-255 / 1,028.
种属反应性
Human, Mouse, Rat
验证应用
IF-Cell, IF-Tissue
分子量
Predicted band size: 109 kDa
阳性对照
Hela, mouse colon tissue.
偶联
iFluor™ 488
克隆号
SD83-03
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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IF-Cell
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1:100
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IF-Tissue
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1:50
靶点
功能
This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
背景文献
1. Kristofik N et al. Impaired von Willebrand factor adhesion and platelet response in thrombospondin-2 knockout mice. Blood 128:1642-50 (2016).
2. Okawa S et al. Lipopolysaccharide induces expression of collagen VI in the rat lung. J Toxicol Pathol 28:37-41 (2015).
亚细胞定位
Extracellular matrix.
UNIPROT
别名
Alpha 1 (VI) chain (61 AA) antibody
CO6A1_HUMAN antibody
COL6A1 antibody
COL6A2 antibody
COL6A3 antibody
Collagen alpha 2(VI) chain antibody
Collagen alpha 3(VI) chain antibody
Collagen alpha-1(VI) chain antibody
Collagen type VI alpha 1 antibody
Collagen type VI alpha 2 antibody
展开图片
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Immunocytochemistry analysis of Hela cells labeling Collagen VI with Rabbit anti-Collagen VI antibody (HA720155F) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-Collagen VI antibody (HA720155F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution. -
Immunofluorescence analysis of paraffin-embedded mouse colon tissue labeling Collagen VI (HA720155F).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Collagen VI (HA720155F, iFluor™ 488) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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