• 概述

• 产品描述 The Cancer Associated Fibroblast Marker Antibody Kit provides an economical means of detecting proteins reported to be expressed in Cancer Associated Fibroblasts (CAFs). The kit includes enough antibodies to perform three western blot experiments with each primary antibody.

• 产品

• 产品包括	数量	应用	反应性	MW(kDa)
  PDGFR alpha[ET1702-49]	30μl	WB,IF-Cell,IHC-P,FC	Human,Mouse,Rat	Predicted band size: 123 kDa
  alpha smooth muscle Actin[ET1607-53]	30μl	WB,IF-Cell,IF-Tissue,IHC-P,FC	Human,Mouse,Rat	42 kDa
  PDGF Receptor beta[ET1605-20]	30μl	WB,IHC-P,IP	Human,Mouse,Rat	Predicted band size:123 kDa
  Vimentin[ET1610-39]	30μl	WB,IF-Cell,IF-Tissue,IHC-P,IP,FC	Human,Mouse,Rat	Predicted band size: 54 kDa
  FAP[ET1704-23]	30μl	WB,IHC-P	Human	88 kDa
  S100A4[ET1612-13]	30μl	WB,IF-Cell,IF-Tissue,IHC-P,IP,FC	Human,Mouse,Rat	12 kDa
  HRP Conjugated Alpaca anti-Rabbit IgG FC, Recombinant VHH[HA1031]	100μl	IP,ELISA,IHC-P,WB	Rabbit	15 kDa

• 性能

• 数据库链接

• 背景介绍

• The tumor microenvironment (TME) has been shown to play an important role in tumor initiation, development, and metastasis. Numerous factors contribute to the nature of the TME such as the presence of immune cells; T-cells, B-cells, and natural killer (NK) cells, and wider environmental factors, such as extracellular matrix (ECM) stiffness, hypoxia, and interstitial pressure. Amongst all these various factors, fibroblasts have been suggested to play a key role in tumor development. Fibroblasts have been studied extensively, however, much regarding their influence on the TME remains to be understood. During tumor development, a subpopulation of hyper-activated fibroblasts become prominent in the TME and secretion of cytokines and chemokines from these cells promotes pro-tumorigenic activity. These highly heterogeneous fibroblast populations are known collectively as CAFs (Cancer Associated Fibroblasts). Due to high plasticity and variability within CAF populations it has been difficult for researchers to define a universal marker for these cells. In lieu of a single marker, a number of markers are currently used to investigate CAFs. PDGFRα and PDGFRβ are common markers used for fibroblast identification, although PDGFRα is more widely expressed over the larger fibroblast populations. α-Smooth Muscle Actin is widely used to identify CAFs, however, some reports suggest it is not expressed by all functionally active CAFs. FSP-1/S100A4 is expressed by cells of mesenchymal origins. Although commonly used as a CAF marker, it too is not expressed by all fibroblasts present in a tumor. Some reports even suggest it to be a marker for quiescent fibroblasts. Fibroblast Activation Protein, or FAP as it is more commonly known, has traditionally been associated with tissue repair, fibrosis, and extracellular matrix degradation. FAP has more recently been described as a useful marker of CAFs. Vimentin strongly characterizes cells of a mesenchymal phenotype. It is frequently used as one marker of CAFs, but it is important to note that it is also highly expressed in fibroblasts of all types, as well as numerous other cell types, such as macrophages and adipocytes, and by epithelial cells undergoing epithelial-to-mesenchymal transition (EMT) .

• 背景文献

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.