p75 NGF Receptor Recombinant Rabbit Monoclonal Antibody [SA39-02] - BSA and Azide free
Catalog# HA750014
p75 NGF Receptor Recombinant Rabbit Monoclonal Antibody [SA39-02] - BSA and Azide free
-
WB
-
IF-Cell
-
IF-Tissue
-
IHC-P
-
IP
-
FC
-
Human
-
Mouse
-
Rat
概述
产品名称
p75 NGF Receptor Recombinant Rabbit Monoclonal Antibody [SA39-02] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human p75 NGF Receptor aa 345-394 / 427.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
分子量
Predicted band size: 45 kDa
阳性对照
Neuro-2a cell lysates, PC-12 cell lysates, Hela, rat uterus tissue, human endometrium tissue, human tonsil tissue, mouse uterus tissue, SH-SY5Y cell lysates, Neuro-2a, PC-12.
偶联
unconjugated
克隆号
SA39-02
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000-1:5,000
-
IF-Cell
-
1:50-1:100
-
IF-Tissue
-
1:50-1:100
-
IHC-P
-
1:200-1:1,000
-
IP
-
1-2μg/sample
-
FC
-
1:200
靶点
功能
The p75 neurotrophin receptor (p75NTR) was first identified in 1973 as the low-affinity nerve growth factor receptor (LNGFR) before discovery that p75NTR bound other neurotrophins equally well as nerve growth factor. p75NTR is a neurotrophic factor receptor. Neurotrophic factor receptors bind Neurotrophins including Nerve growth factor, Neurotrophin-3, Brain-derived neurotrophic factor, and Neurotrophin-4. All neurotrophins bind to p75NTR. This also includes the immature pro-neurotrophin forms. Neurotrophic factor receptors, including p75NTR, are responsible for ensuring a proper density to target ratio of developing neurons, refining broader maps in development into precise connections. p75NTR is involved in pathways that promote neuronal survival and neuronal death.
背景文献
1. Blanchard JW et al. Selective conversion of fibroblasts into peripheral sensory neurons. Nat Neurosci 18:25-35 (2015).
2. Fernandez-Enright F et al. Novel implications of Lingo-1 and its signaling partners in schizophrenia. Transl Psychiatry 4:e348 (2014).
翻译后修饰
N- and O-glycosylated.; O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.; Phosphorylated on serine residues.
亚细胞定位
Membrane
别名
CD271 antibody
CD271 antigen antibody
Gp80 LNGFR antibody
Gp80-LNGFR antibody
Low affinity nerve growth factor receptor antibody
Low affinity neurotrophin receptor p75NTR antibody
Low-affinity nerve growth factor receptor antibody
Nerve growth factor receptor antibody
Nerve growth factor receptor TNFR superfamily member 16 antibody
NGF receptor antibody
展开图片
-
Western blot analysis of p75 NGF Receptor on Neuro-2a cell lysates with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/2,000 dilution.
Lysates/proteins at 15 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 70 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750014) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of p75 NGF Receptor on PC-12 cell lysates with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/5,000 dilution.
Lysates/proteins at 15 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 70 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750014) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of p75 NGF Receptor on different lysates with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/2,000 dilution.
Lane 1: PC-12-si NT cell lysate
Lane 2: PC-12-si p75 NGF Receptor cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 70 kDa
Exposure time: 2 minutes 6 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750014) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of Hela cells labeling p75 NGF Receptor with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Immunohistochemical analysis of paraffin-embedded rat uterus tissue with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750014) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750014) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750014) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-p75 NGF Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750014, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Flow cytometric analysis of PC-12 cells labeling p75 NGF Receptor.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA750014, 1:200) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Western blot analysis of p75 NGF Receptor on SH-SY5Y cell lysates with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/2,000 dilution.
Lysates/proteins at 20µg/Lane.
Predicted band size: 45 kDa
Observed band size: 70 kDa
Exposure time: 1 minutes 59 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750014) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of Neuro-2a cells labeling p75 NGF Receptor with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of PC-12 cells labeling p75 NGF Receptor with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
p75 NGF Receptor was immunoprecipitated from 0.2 mg C6 cell lysate with HA750014 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750014 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: C6 cell lysate (input)
Lane 2: HA750014 IP in C6 cell lysate
Lane 3: Rabbit IgG instead of HA750014 in C6 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 20 seconds; ECL: K1801
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
iFluor™ 488 Conjugated p75 NGF Receptor Recombinant Rabbit Monoclonal Antibody [SA39-02]
Application: IF-Cell
Reactivity: Human,Mouse,Rat
Conjugate: iFluor™ 488
