VIL1 Mouse Monoclonal Antibody [G11-5E]
Catalog# EM1706-46
VIL1 Mouse Monoclonal Antibody [G11-5E]
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WB
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IF-Cell
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IHC-P
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FC
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Human
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unconjugated
概述
产品名称
VIL1 Mouse Monoclonal Antibody [G11-5E]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein
种属反应性
Human
验证应用
WB, IF-Cell, IHC-P, FC
分子量
93 kDa
阳性对照
Human VIL1 recombinant protein, VIL1-hIgGFc transfected HEK293 cell lysate, SW620, Hela, human cervical cancer tissue.
偶联
unconjugated
克隆号
G11-5E
产品特性
形态
Liquid
浓度
存放说明
4℃; -20℃ for long term storage.
存储缓冲液
Purified antibody in PBS with 0.05% sodium azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:2,000
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IF-cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
靶点
功能
Caldesmon, Filamin 1, Nebulin and Villin are differentially expressed and regulated Actin binding proteins. Both muscular (CDh) and non-muscular (CDl) forms of Caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and myosin. CDh is expressed predominantly on thin filaments in smooth muscle, whereas CDl is widely expressed in non-muscle tissues and cells. Filamin 1, which is ubiquitously expressed and exists as a homodimer, functions to crosslink Actin to filaments. Nebulin is a large filamentous protein specific to muscle tissue that may function as a ruler for filament length. Several isoforms of Nebulin are produced by alternative exon usage. Villin is Ca2+-regulated and is the major structural component of the brush border of absorptive cells.
背景文献
1. Xieraili M et al. Villin 1 is a predictive factor for the recurrence of high serum alpha-fetoprotein-associated hepatocellular carcinoma after hepatectomy. Cancer Sci 103(8):1493-501 (2012).
2. Nakamura E et al. Villin1, a diagnostic marker for endometrial adenocarcinoma with high grade nuclear atypia. Cancer Biol Ther 1;12(3):181-90 (2011).
亚细胞定位
Cytoplasm, cytoskeleton.
UNIPROT
别名
D2S1471 antibody
OTTHUMP00000164145 antibody
VIL antibody
VIL1 antibody
VILI_HUMAN antibody
Villin 1 antibody
Villin-1 antibody
Villin1 antibody
图片
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Western blot analysis of Villin on human Villin recombinant protein using anti- Villin antibody at 1/1,000 dilution.
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Western blot analysis of Villin on HEK293 (1) and Villin -hIgGFc transfected HEK293 (2) cell lysate using anti- Villin antibody at 1/1,000 dilution.
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Western blot analysis of Villin on SW620 cell lysate using anti- Villin antibody at 1/1,000 dilution.
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ICC staining Villin (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti- Villin antibody. Counter stained with hematoxylin.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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