TRAF3 Recombinant Rabbit Monoclonal Antibody [PSH01-30]
Catalog# HA721654
TRAF3 Recombinant Rabbit Monoclonal Antibody [PSH01-30]
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WB
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Human
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Mouse
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Rat
概述
产品名称
TRAF3 Recombinant Rabbit Monoclonal Antibody [PSH01-30]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human TRAF3 301-568 / 568.
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 64 kDa
阳性对照
TF-1 cell lysate, THP-1 cell lysate, 293T cell lysate, Jurkat cell lysate, A549 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, mouse brain tissue lysate, rat spleen tissue lysate.
偶联
unconjugated
克隆号
PSH01-30
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
靶点
功能
TNF receptor-associated factor (TRAF3) is a protein that in humans is encoded by the TRAF3 gene. The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from, members of the TNF receptor (TNFR) superfamily. This protein participates in the signal transduction of CD40, a TNFR family member important for the activation of the immune response. This protein is found to be a critical component of the lymphotoxin-beta receptor (LTbetaR) signaling complex, which induces NF-kappaB activation and cell death initiated by LTbeta ligation. Epstein-Barr virus-encoded latent infection membrane protein-1 (LMP1) can interact with this and several other members of the TRAF family, which may be essential for the oncogenic effects of LMP1. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.
背景文献
1. Gao P et al. E3 ligase Nedd4l promotes antiviral innate immunity by catalyzing K29-linked cysteine ubiquitination of TRAF3. Nat Commun. 2021 Feb
2. Sun N et al. TRIM35 mediates protection against influenza infection by activating TRAF3 and degrading viral PB2. Protein Cell. 2020 Dec
亚细胞定位
Cytoplasm, Endosome, Mitochondrion.
别名
CAP 1 antibody
CAP-1 antibody
CAP1 antibody
CD40 associated protein 1 antibody
CD40 binding protein antibody
CD40 bp antibody
CD40 receptor associated factor 1 antibody
CD40 receptor-associated factor 1 antibody
CD40-binding protein antibody
CD40BP antibody
展开图片
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Western blot analysis of TRAF3 on different lysates with Rabbit anti-TRAF3 antibody (HA721654) at 1/1,000 dilution.
Lane 1: TF-1 cell lysate (20 µg/Lane)
Lane 2: THP-1 cell lysate (20 µg/Lane)
Lane 3: 293T cell lysate (20 µg/Lane)
Lane 4: Jurkat cell lysate (20 µg/Lane)
Lane 5: A549 cell lysate (20 µg/Lane)
Lane 6: RAW264.7 cell lysate (20 µg/Lane)
Lane 7: NIH/3T3 cell lysate (20 µg/Lane)
Lane 8: Mouse brain tissue lysate (40 µg/Lane)
Lane 9: Rat spleen tissue lysate (40 µg/Lane)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 64 kDa
Observed band size: 50 kDa
Exposure time: 1 minute 9 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721654) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
TRAF3 Rabbit Polyclonal Antibody
Application: WB
Reactivity: Human,Mouse
Conjugate: unconjugated
