SAP97 Recombinant Rabbit Monoclonal Antibody [JE39-74]
Catalog# HA721502
SAP97 Recombinant Rabbit Monoclonal Antibody [JE39-74]
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WB
-
IHC-P
-
FC
-
Human
-
Mouse
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Rat
概述
产品名称
SAP97 Recombinant Rabbit Monoclonal Antibody [JE39-74]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within N terminal Human SAP97.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, FC
分子量
Predicted band size: 100 kDa
阳性对照
MCF7 cell lysate, HepG2 cell lysate, U-87 MG cell lysate, SiHa cell lysate, NCI-H226 cell lysate, NIH/3T3 cell lysate, mouse liver tissue, rat cerebellum tissue, SiHa.
偶联
unconjugated
克隆号
JE39-74
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
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IHC-P
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1:200
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FC
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1:500-1:1,000
靶点
功能
Discs large homolog 1 (DLG1), also known as synapse-associated protein 97 or SAP97, is a scaffold protein that in humans is encoded by the SAP97 gene. SAP97 is a mammalian MAGUK-family member protein that is similar to the Drosophila protein Dlg1 (the protein is alternatively referred to as hDlg1, and the human gene is DLG1). SAP97 is expressed throughout the body in epithelial cells. In the brain it is involved in the trafficking of ionotropic receptors from the endoplasmic reticulum to the plasma membrane, and may be involved in the trafficking AMPAR during synaptic plasticity. SAP97 is expressed throughout the body in epithelial cells, including the kidney and brain. There is some evidence that SAP97 regulates cell-to-cell adhesion during cell death, and may interact with HPV. In the brain, SAP97's function is involved in the trafficking of transmembrane receptors from the ER to the plasma membrane. SAP97's function has been investigated by reducing its expression by knockout or increasing its expression heterologously. Mice in which the SAP97 gene has been knocked out die perinatally, have a cleft palate, and deficiencies in renal function. Overexpression of SAP97 in mammalian neurons leads to increased synaptic strength.
背景文献
1. Bahouth SW et al. Involvement of SAP97 anchored multiprotein complexes in regulating cardiorenal signaling and trafficking networks. Biochem Pharmacol. 2023 Feb
2. Xu X et al. SAP97 polymorphisms associated with early onset Parkinson\'s disease. Neurosci Lett. 2020 May
亚细胞定位
Membrane, Basolateral cell membrane, Endoplasmic reticulum membrane, Postsynaptic density, Synapse, Cell membrane, sarcolemma, Apical cell membrane, Cell junction, Cytoplasm.
别名
Discs large homolog 1 antibody
discs large, Drosophila, homolog of, 1 antibody
discs, large homolog 1 (Drosophila) antibody
Disks large homolog 1 antibody
dJ1061C18.1.1 antibody
DKFZp761P0818 antibody
DKFZp781B0426 antibody
DLG 1 antibody
DLG1 antibody
DLG1_HUMAN antibody
展开图片
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Western blot analysis of SAP97 on different lysates with Rabbit anti-SAP97 antibody (HA721502) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: U-87 MG cell lysate
Lane 4: SiHa cell lysate
Lane 5: NCI-H226 cell lysate
Lane 6: NIH/3T3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 100 kDa
Observed band size: 120 kDa
Exposure time: 1 minute 55 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721502) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of SAP97 on different lysates with Rabbit anti-SAP97 antibody (HA721502) at 1/2,000 dilution.
Lane 1: A549-WT cell lysate
Lane 2: A549-KD SAP97 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 100 kDa
Observed band size: 120 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721502) at 1/2,000 dilution was used in 2% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-SAP97 antibody (HA721502) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721502) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-SAP97 antibody (HA721502) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721502) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of SiHa cells labeling SAP97.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721502, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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