Phospho-GSK3 alpha (S21) Recombinant Rabbit Monoclonal Antibody [PSH09-11]
概述
产品名称
Phospho-GSK3 alpha (S21) Recombinant Rabbit Monoclonal Antibody [PSH09-11]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic phospho-peptide corresponding to residues surrounding Ser21 of Human GSK3 alpha.
种属反应性
Human, Mouse
验证应用
WB, IHC-P, Dot Blot
分子量
Predicted band size: 51 kDa
阳性对照
A549 treated with 100nM Calyculin A for 30 minutes cell lysate, SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate, NIH/3T3 starved overnight cell lysate, NIH/3T3 starved overnight then treated with 100ng/mL PDGF for 5 minutes cell lysate, mouse testis tissue, human breast tissue, human colon cancer tissue.
偶联
unconjugated
克隆号
PSH09-11
反应性数据
| WB | IHC-P | Dot Blot | |
|---|---|---|---|
| Human |
|
|
|
| Mouse |
|
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
-
IHC-P
-
1:200-1:500
-
Dot Blot
-
1:1,000
靶点
功能
Glycogen synthase kinase-3 alpha is an enzyme that in humans is encoded by the GSK3A gene. Glycogen synthase kinase 3-alpha EC 2.7.1.37 is a multifunctional protein serine kinase, homologous to Drosophila 'shaggy' (zeste-white3) and implicated in the control of several regulatory proteins including glycogen synthase and various transcription factors (e.g., JUN). It also plays a role in the WNT and phosphoinositide 3-kinase (especially PIK3CG) signaling pathways.
背景文献
1. Wang J et al. Quantitative phosphoproteomics reveals GSK3A substrate network is involved in the cryodamage of sperm motility. Biosci Rep. 2021 Oct
2. Zhang W et al. Active Ingredient Paeonol of Jijiu Huiyang Decoction Alleviates Isoproterenol-Induced Chronic Heart Failure via the GSK3A/PPARalpha Pathway. Oxid Med Cell Longev. 2023 Feb
亚细胞定位
Apical dendrite, axon, cytoplasm, cytosol, mitochondrion, neuronal cell body, nucleus, postsynapse, proximal dendrite.
别名
DKFZp686D0638 antibody
Glycogen synthase kinase 3 alpha antibody
Glycogen synthase kinase-3 alpha antibody
GSK 3 alpha antibody
GSK 3A antibody
GSK-3 alpha antibody
Gsk3a antibody
GSK3A_HUMAN antibody
GSK3alpha antibody
Serine/threonine protein kinase GSK3A antibody
图片
-
☑ Cell treatment (CT)
Western blot analysis of Phospho-GSK3 alpha (S21) on different lysates with Rabbit anti-Phospho-GSK3 alpha (S21) antibody (HA723069) at 1/2,000 dilution.
Lane 1: A549 cell lysate
Lane 2: A549 treated with 100nM Calyculin A for 30 minutes cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723069) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of Phospho-GSK3 alpha (S21) on different lysates with Rabbit anti-Phospho-GSK3 alpha (S21) antibody (HA723069) at 1/2,000 dilution.
Lane 1: NIH/3T3 starved overnight cell lysate
Lane 2: NIH/3T3 starved overnight then treated with 100ng/mL PDGF for 5 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723069) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Phospho-GSK3 alpha (S21) antibody (HA723069) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723069) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Phospho-GSK3 alpha (S21) antibody (HA723069) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723069) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Phospho-GSK3 alpha (S21) antibody (HA723069) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723069) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Dot blot analysis of Phospho-GSK3 alpha (S21) on different peptides with Rabbit anti-Phospho-GSK3 alpha (S21) antibody (HA723069) at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution for 1 hour at room temperature.
Lane 1: Phospho-GSK3 alpha (S21) peptide (positive)
Lane 2: Unmodified GSK3 alpha peptide (negative)
Lane 3: Phospho-GSK3 beta (S9) peptide (negative)
Lane 4: Unmodified GSK3 beta peptide (negative)
Proteins loading: 100ng, 25ng, 5ng;
Blocking and dilution buffer: 5% NFDM/TBST;
Exposure time: 10 seconds; ECL: K1801.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
Swietenine improved the progression of diabetic nephropathy through inhibiting ferroptosis via activating Akt/GSK-3β/Nrf2 signaling pathway
期刊: Journal Of Ethnopharmacology
DOI: 10.1016/j.jep.2025.119981
IF: 4.8
应用: WB
反应种属: Mouse
发表时间: 2025 May
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