Phospho-EGFR (Y1092) Recombinant Rabbit Monoclonal Antibody [SJ0194]
概述
产品名称
Phospho-EGFR (Y1092) Recombinant Rabbit Monoclonal Antibody [SJ0194]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic phospho-peptide corresponding to residues surrounding Tyr1092 of Human EGFR aa 1,071-1,120 / 1,210.
种属反应性
Human, Mouse
验证应用
WB, IF-Cell, IF-Tissue, IHC-P
分子量
170 kDa
阳性对照
A431 cell lysate treated with EGF, BT-20, human tonsil tissue, mouse skin tissue.
偶联
unconjugated
克隆号
SJ0194
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000-1:2,000
-
IF-Cell
-
1:50-1:200
-
IF-Tissue
-
1:50-1:200
-
IHC-P
-
1:50-1:200
发表文章中的应用
靶点
功能
Epidermal growth factor mediates its effects on cell growth through its inter-action with a cell surface glycoprotein designated the EGF receptor. Binding of EGF or TGF alpha to the EGF receptor activates tyrosine-specific protein kinase activity intrinsic to the EGF receptor. The carboxy terminal tyrosine residues on EGFR, Tyr 1068 and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding. Once activated, EGFR mediates the binding of the phosphotyrosine binding (PTB) domain of GRB2 through direct interactions with Tyr 1068 and Tyr 1086 and through indirect interactions with Tyr 1173 in the Ras signaling pathway. Tyr 1173 of EGFR also functions as a kinase substrate. Phosphorylation of Tyr 992, Tyr 1068 and Tyr 1086 is required for conformational change in the C-terminal tail of the EGF receptor.
背景文献
1. Nakamura T et al. LRIG1 inhibits STAT3-dependent inflammation to maintain corneal homeostasis. J Clin Invest 124:385-97 (2014).
2. Furcht CM et al. Multivariate signaling regulation by SHP2 differentially controls proliferation and therapeutic response in glioma cells. J Cell Sci 127:3555-67 (2014).
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
组织特异性
Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
翻译后修饰
Phosphorylated on Tyr residues in response to EGF. Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.; Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126 (By similarity).; Palmitoylated on Cys residues by ZDHHC20. Palmitoylation inhibits internalization after ligand binding, and increases the persistence of tyrosine-phosphorylated EGFR at the cell membrane. Palmitoylation increases the amplitude and duration of EGFR signaling.; Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.
亚细胞定位
Cell membrane, Nucleus membrane, Nucleus, Endoplasmic reticulum membrane, Golgi apparatus membrane, Endosome.
UNIPROT #
别名
Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
Cell growth inhibiting protein 40 antibody
Cell proliferation inducing protein 61 antibody
EGF R antibody
EGFR antibody
EGFR_HUMAN antibody
Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
Epidermal growth factor receptor antibody
erb-b2 receptor tyrosine kinase 1 antibody
展开Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
Cell growth inhibiting protein 40 antibody
Cell proliferation inducing protein 61 antibody
EGF R antibody
EGFR antibody
EGFR_HUMAN antibody
Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
Epidermal growth factor receptor antibody
erb-b2 receptor tyrosine kinase 1 antibody
ERBB antibody
ERBB1 antibody
Errp antibody
HER1 antibody
mENA antibody
NISBD2 antibody
Oncogen ERBB antibody
PIG61 antibody
Proto-oncogene c-ErbB-1 antibody
Receptor tyrosine protein kinase ErbB 1 antibody
Receptor tyrosine-protein kinase ErbB-1 antibody
SA7 antibody
Species antigen 7 antibody
Urogastrone antibody
v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
wa2 antibody
Wa5 antibody
折叠图片
-
Western blot analysis of Phospho-EGFR (Y1092) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1606-44, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A431 cell lysate treated with EGF
Lane 2: Untreated A431 cell lysate -
Western blot analysis of Phospho-EGFR(Y1092) on A431 cell lysates.
Lane 1: A431 cells, whole cell lysate, 10ug/lane
Lane 2/3: A431 cells treated with 100 ng/ml EGF for 30 minutes, whole cell lysates, 10ug/lane
Lane 4: A431 cells treated with 100 ng/ml EGF for 30 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane
All lanes :
Anti-Phospho-EGFR(Y1092) antibody (ET1606-44
) at 1:500 dilution. Anti-EGFR antibody (ET1603-37) at 1:500 dilution. Anti-Hsp90 beta antibody (ET1605-56) at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution.
Predicted band size: 134 kDa
Observed band size: 170 kDa
Blocking and diluting buffer: 5% BSA.
Exposure time: 15 seconds -
ICC staining of Phospho-EGFR (Y1092) in BT-20 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1606-44, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
-
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-EGFR (Y1092) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-44, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Phospho-EGFR (Y1092) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-44, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-Phospho-EGFR (Y1092) antibody (ET1606-44) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-44) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
Daidzein alleviates osteoporosis by promoting osteogenesis and angiogenesis coupling
Author:
PMID: 37868048
应用: WB,IF
反应种属: Mouse
发表时间: 2023 Oct
-
Citation
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The role of EGFR signaling in age-related osteoporosis in mouse cortical bone
Author: Liu, G., Xie, Y., Su, J., Qin, H., Wu, H., Li, K., Yu, B., & Zhang, X.
PMID: 31298955
应用: WB,IF
反应种属: Mouse
发表时间: 2019 Oct
-
Citation