Phospho-BTK (Y223) Recombinant Rabbit Monoclonal Antibody [ST51-05]
Catalog# ET1609-55
Phospho-BTK (Y223) Recombinant Rabbit Monoclonal Antibody [ST51-05]
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WB
-
IP
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Human
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Mouse
预测的种属支持售后
概述
产品名称
Phospho-BTK (Y223) Recombinant Rabbit Monoclonal Antibody [ST51-05]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic phospho-peptide corresponding to residues surrounding Tyr223 of human BTK.
种属反应性
Human (Predicted: Mouse)
预测的种属支持售后
验证应用
WB, IP
分子量
Predicted band size: 76 kDa
阳性对照
Ramos cell lysate, Ramos treated with 12μg/mL human IgM for 10 minutes cell lysate, K562 cells treated with pervanadate.
偶联
unconjugated
克隆号
ST51-05
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000-1:2,000
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IP
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Use at an assay dependent concentration.
靶点
功能
The Tec family of non-receptor tyrosine kinases is composed of six proteins designated Tec, Emt (also known as Itk or Tsk), Btk (previously known as Atk, BPK or Emb), Bmx, Txk (also known as Rlk) and Dsrc28C. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini. Four alternatively spliced forms of Tec are found to be expressed broadly in cells of hematopoietic lineage and hepatocytes. The Emt gene product associates with CD28 and becomes activated subsequent to CD28 ligation. Btk is necessary for proper B cell development, and mutations in the gene encoding Btk have been associated with families suffering from X-linked agammaglobulinemia, also referred to as Bruton’s disease. The Bmx protein shares a high degree of homology with Btk and seems to be expressed at highest levels in the heart. Txk expression is T cell-specific, while expression of the Drosophila Tec homolog, Dsrc28C, is developmentally regulated.
背景文献
1. Guo W et al. Targeting Btk/Etk of prostate cancer cells by a novel dual inhibitor. Cell Death Dis 5:e1409 (2014).
2. Dong S et al. IPI-145 antagonizes intrinsic and extrinsic survival signals in chronic lymphocytic leukemia cells. Blood 124:3583-6 (2014).
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
组织特异性
Predominantly expressed in B-lymphocytes.
翻译后修饰
Following B-cell receptor (BCR) engagement, translocates to the plasma membrane where it gets phosphorylated at Tyr-551 by LYN and SYK. Phosphorylation at Tyr-551 is followed by autophosphorylation of Tyr-223 which may create a docking site for a SH2 containing protein. Phosphorylation at Ser-180 by PRKCB, leads in translocation of BTK back to the cytoplasmic fraction. Phosphorylation at Ser-21 and Ser-115 creates a binding site for PIN1 at these Ser-Pro motifs, and promotes it's recruitment.
亚细胞定位
Cytoplasm, Nucleus, Cell membrane.
别名
Agammaglobulinaemia tyrosine kinase antibody
AGMX 1 antibody
AGMX1 antibody
AT antibody
ATK antibody
B cell progenitor kinase antibody
B-cell progenitor kinase antibody
BPK antibody
Bruton agammaglobulinemia tyrosine kinase antibody
Bruton tyrosine kinase antibody
展开图片
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☑ Cell treatment (CT)
Western blot analysis of Phospho-BTK (Y223) on different lysates with Rabbit anti-Phospho-BTK (Y223) antibody (ET1609-55) at 1/1,000 dilution.
Lane 1: Ramos cell lysate
Lane 2: Ramos treated with 12μg/mL human IgM for 10 minutes cell lysate
Lane 3: Ramos treated with 12μg/mL human IgM for 10 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 76 kDa
Observed band size: 76 kDa
Exposure time: 59 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-55) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of Phospho-BTK(Y223) on different lysates using anti-Phospho-BTK(Y223) antibody at 1/1,000 dilution.
Positive control:
Lane 1: K562 cells treated with pervanadate
Lane 2: Untreated K562 cell lysate
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
BTK Recombinant Rabbit Monoclonal Antibody [SN69-07] - BSA and Azide free
Application: WB,IHC-P,IP
Reactivity: Human,Mouse
Conjugate: unconjugated
