Phospho-4E-BP1/2/3 (T45) Mouse Monoclonal Antibody [3G2]
Catalog# RT1004
Phospho-4E-BP1/2/3 (T45) Mouse Monoclonal Antibody [3G2]
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WB
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IP
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IF
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Human
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Mouse
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Rat
预测的种属支持售后
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unconjugated
概述
产品名称
Phospho-4E-BP1/2/3 (T45) Mouse Monoclonal Antibody [3G2]
抗体类型
Mouse Monoclonal Antibody
免疫原
Synthetic peptide.
种属反应性
Human (Predicted: Mouse, Rat)
预测的种属支持售后
验证应用
WB, IP, IF
分子量
21 kDa
阳性对照
293T cell lysates.
偶联
unconjugated
克隆号
3G2
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃
存储缓冲液
1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
亚型
IgG2a
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:100-1:1,000
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IP
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1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
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IF
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1:50-500
靶点
功能
The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits to the 5' end of mRNA. The eIF4F subunit eIF4E interacts directly with the mRNA 5' cap structure. Assembly of the eIF4F complex is inhibited by a family of repressor polypeptides, the eIF4E-binding proteins (4E-BPs). 4E-BP1 (also known as PHAS-1) normally binds eIF4E, inhibiting cap-dependent translation. Hyper-phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. The PI3-kinase/Akt pathway and the FRAP/mTOR kinase regulate 4E-BP1. 4E-BP1 is phosphorylated in vivo on multiple residues and phosphorylation by FRAP/mTOR on Threonine 37 and Threonine 46 of human 4E-BP1 may prime it for sub-sequent phosphorylation at sites including Serine 65 and Threonine 70. The corresponding rat residues include Threonine 36, Threonine 45, Serine 64 and Threonine 69. In vitro, 4E-BP1 is also phosphorylated by ataxia telangiectasia (ATM) at human Serine 112 (rat Serine 111) in response to an increase in insulin levels.
背景文献
1. Yang, D.Q., et al. 2000. Participation of ATM in Insulin signalling through phosphorylation of eIF-4E-binding protein 1. Nat. Cell Biol. 2: 893-898.
2. Gingras, A.C., et al. 1999. Regulation of 4E-BP1 phosphorylation: a novel two-step mechanism. Genes Dev. 13: 1422-1437.
3. Gingras, A.C., et al. 1998. 4E-BP1, a repressor of mRNA translation, is phosphorylated and inactivated by the Akt (PKB) signaling pathway. Genes Dev. 12: 502-513.
亚细胞定位
Cytoplasm.
别名
4EBP1 antibody
4EBP2 antibody
4EBP3 antibody
eIF4E binding protein 1 antibody
eIF4E binding protein 2 antibody
eIF4E binding protein 3 antibody
Eukaryotic translation initiation factor 4E binding protein 1 antibody
Eukaryotic translation initiation factor 4E binding protein 2 antibody
Eukaryotic translation initiation factor 4E binding protein 3 antibody
PHAS1 antibody
图片
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Westernblot analysis of 4E-BP1/2/3 phosphorylation inuntreated (A), calyculinA treated (B) and calyculin A and lambda protein phosphatase treated (C) Jurkat whole cell lysates.
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Western blot analysis of 4E-BP1 phosphorylation in nontransfected (A,D), untreated human 4E-BP1 transfected (B,E) and lambda protein phosphatase treated human 4E-BP1 transfected (C,F) 293T whole cell lysates. Antibodies tested include p-4E-BP1/2/3 (A,B,C) and 4E-BP1 (D,E,F).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Podophyllum hexandrum Royle mitigates perimenopausal symptoms in an OVX rat model by activating the PI3K/AKT/mTOR pathway and enhancing estrogen receptor expression
期刊: Journal Of Ethnopharmacology
DOI: 10.1016/j.jep.2025.119968
IF: 4.8
应用: WB
反应种属: Rat
发表时间: 2025 May
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Effects of electrolyte balance on intestinal barrier, amino acid metabolism, and mTORC1 signaling pathway in piglets fed low-protein diets
期刊: Animal Nutrition
DOI: 10.1016/j.aninu.2024.03.011
IF: 6.1
应用: WB
反应种属: Pig
发表时间: 2024 Mar
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Glucocorticoid Regulates the Synthesis of Porcine Muscle Protein through m6A Modified Amino Acid Transporter SLC7A7
期刊: International Journal Of Molecular Sciences
DOI:
IF: 5.922
应用: WB
反应种属: Pig
发表时间: 2022 Jan
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